Goat Pyruvate dehydrogenase Polyclonal Antibody

Pyruvate dehydrogenase (PDHX, E3BP, OPDX, PDX1, proX, DLDBP, pyruvate dehydrogenase complex, component X, E3-binding protein, pyruvate dehydrogenase complex, lipoyl-containing component X)

Cat. Num. AAA620239

• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Pyruvate dehydrogenase; Polyclonal Antibody; Pyruvate dehydrogenase (PDHX; E3BP; OPDX; PDX1; proX; DLDBP; pyruvate dehydrogenase complex; component X; E3-binding protein; lipoyl-containing component X); Anti -Pyruvate dehydrogenase (PDHX; anti-Pyruvate dehydrogenase antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Pyruvate dehydrogenase at ~50kD. Species Sequence Homology: mouse, rat, canine
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-Pyruvate dehydrogenase antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA (Peptide): 1:32,000; Western Blot: 0.1-1ug/ml ~50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293.; Immunohistochemistry (Paraffin): 1-3ug/ml Shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells in human pancreas.
• Immunogen: Synthetic peptide, C-KSFKANLENPIRLA from the C terminus of human Pyruvate dehydrogenase.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Chemiluminescence

(MBS620239 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

Immunohistochemistry (IHC)

(MBS620239 staining of paraffin embedded Human Pancreas. A) Microwaved antigen retrieval with citrate buffer pH 6 (3ug/ml), HRP-staining. B) Microwaved antigen retrieval with Tris/EDTA buffer pH9 (1ug/ml) , HRP-staining.)

Related Product Information for anti-Pyruvate dehydrogenase antibody

The pyruvate dehydrogenase (PDH) complex is located in the mitochondrial matrix and catalyzes the conversion of pyruvate to acetyl coenzyme A. The PDH complex thereby links glycolysis to Krebs cycle. The PDH complex contains three catalytic subunits, E1, E2, and E3, two regulatory subunits, E1 kinase and E1 phosphatase, and a non-catalytic subunit, E3 binding protein (E3BP). This gene encodes the E3 binding protein subunit; also known as component X of the pyruvate dehydrogenase complex. This protein tethers E3 dimers to the E2 core of the PDH complex. Defects in this gene are a cause of pyruvate dehydrogenase deficiency which results in neurological dysfunction and lactic acidosis in infancy and early childhood. This protein is also a minor antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC eventually leads to cirrhosis and liver failure. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Product Categories/Family for anti-Pyruvate dehydrogenase antibody

Antibodies; Abs to Protein Kinase Substrates

NCBI and Uniprot Product Information

• NCBI GI #: 300763221
• NCBI Official Full Name: pyruvate dehydrogenase (lipoamide) E1 component, beta subunit; pyruvate decarboxylase.; pyruvate dehydrogenase.; Pyruvic dehydrogenase
• Protein Family: Pyruvate dehydrogenase