Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: 862371(TRIM71 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Rabbit anti-Mouse TRIM71/LIN41 Polyclonal Antibody | anti-TRIM71/LIN41 antibody
TRIM71/LIN41 Rabbit pAb
Gene Names
TRIM71; LIN41; LIN-41
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity purified
Synonyms
TRIM71/LIN41; Polyclonal Antibody; TRIM71/LIN41 Rabbit pAb; LIN41; LIN-41; anti-TRIM71/LIN41 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-TRIM71/LIN41 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:1000-1:5000
Immunogen
Synthetic peptide of human TRIM71
Conjugation
Unconjugated
Molecular Function
LIN-41, also called tripartite motif-containing 71 (TRIM71), which was first identified in Caenorhabditis elegans, is responsible for the timing of cell fate determination. By encoding microRNAs (miRNAs), the heterochronic genes let-7 and lin-4 downregulate the gene encoding LIN-41. The miRNAs bind to six complementary sites on the 3' untranslated region (UTR) of the LIN-41 gene. This downregulation positively regulates the timing of the expression of LIN-29, an adult specification transcription factor.
Modification
Unmodified
Cell Position
Cytoplasm
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: 862371(TRIM71 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Western Blot (WB)
(Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: 862371(TRIM71 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds)
Product Categories/Family for anti-TRIM71/LIN41 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
93,385 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase TRIM71
NCBI Official Synonym Full Names
tripartite motif containing 71, E3 ubiquitin protein ligase
NCBI Official Symbol
TRIM71
NCBI Official Synonym Symbols
LIN41; LIN-41
NCBI Protein Information
E3 ubiquitin-protein ligase TRIM71; protein lin-41 homolog; abnormal cell LINeage LIN-41; homolog of C. elegans Lin-41; tripartite motif-containing 71; tripartite motif-containing protein 71
UniProt Protein Name
E3 ubiquitin-protein ligase TRIM71
UniProt Gene Name
TRIM71
UniProt Synonym Gene Names
LIN41
UniProt Entry Name
LIN41_HUMAN
Uniprot Description
TRIM71: May be involved in controlling the timing of organ formation during development. Belongs to the TRIM/RBCC family.
Chromosomal Location of Human Ortholog: 3p22.3
Molecular Function: miRNA binding; zinc ion binding; ubiquitin-protein ligase activity; ligase activity
Biological Process: neural tube development; protein autoubiquitination; fibroblast growth factor receptor signaling pathway; neural tube closure; miRNA-mediated gene silencing, negative regulation of translation; G1/S transition of mitotic cell cycle
Research Articles on TRIM71/LIN41
Similar Products
Product Notes
The TRIM71/LIN41 trim71 (Catalog #AAA4758560) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIM71/LIN41 Rabbit pAb reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM71/LIN41 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:1000-1:5000. Researchers should empirically determine the suitability of the TRIM71/LIN41 trim71 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRIM71/LIN41, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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