Rabbit TRIM69 Polyclonal Antibody | anti-TRIM69 antibody

TRIM69 Polyclonal Antibody

Cat. Num. AAA3011375

• Gene Names: TRIM69; Trif; HSD34; RNF36; HSD-34
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TRIM69; Polyclonal Antibody; TRIM69 Polyclonal Antibody; E3 ubiquitin protein ligase TRIM69; HSD34; RFP like domain containing protein trimless; RFP-like domain-containing protein trimless; RING finger protein 36; RNF36; TRI69_HUMAN; Trif; Trim69; Trimless; Tripartite motif containing 69; tripartite motif containing protein 69; tripartite motif protein 69; Tripartite motif-containing protein 69; anti-TRIM69 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRIM69 polyclonal antibody detects endogenous levels of TRIM69 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 500

• Applicable Applications for anti-TRIM69 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant full length Human TRIM69.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TRIM69 polyclonal antibody)

Related Product Information for anti-TRIM69 antibody

-The tripartite motif (TRIM) family of proteins are characterized by a conserved TRIM domain that includes a coiled-coil region, a B-box type zinc finger, one RING finger and three zinc-binding domains. TRIM69 (tripartite motif-containing 69), also known as Trif, HSD34 or RNF36 (RING finger protein 36), is a 500 amino acid protein that belongs to the TRIM family and contains one RING-type zinc finger and one B30.2/SPRY domain. Localizing to nuclear speckles, TRIM69 interacts with PML (promyelocytic leukemia) and is thought to play a role in spermatogenesis and, when overexpressed, may be involved in apoptosis. TRIM69 is subject to posttranslational phosphorylation, an event which is necessary for TRIM69 nuclear localization. Multiple isoforms of TRIM69 exist due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 88999601
• NCBI GeneID: 140691
• NCBI Accession #: NP_892030.3
• NCBI GenBank Nucleotide #: NP_892030.3
• UniProt Accession #: Q86WT6
• Molecular Weight: ~ 57kDa
• NCBI Official Full Name: E3 ubiquitin-protein ligase TRIM69 isoform a
• NCBI Official Synonym Full Names: tripartite motif containing 69
• NCBI Official Symbol: TRIM69
• NCBI Official Synonym Symbols: Trif; HSD34; RNF36; HSD-34
• NCBI Protein Information: E3 ubiquitin-protein ligase TRIM69
• UniProt Protein Name: E3 ubiquitin-protein ligase TRIM69
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: TRIM69
• UniProt Synonym Gene Names: RNF36
• UniProt Entry Name: TRI69_HUMAN

NCBI Description

This gene encodes a member of the RING-B-box-coiled-coil (RBCC) family and encodes a protein with an N-terminal RING finger motif, a PRY domain and a C-terminal SPRY domain. The mouse ortholog of this gene is specifically expressed in germ cells at the round spermatid stages during spermatogenesis and, when overexpressed, induces apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

TRIM69: May play a role in apoptosis. Belongs to the TRIM/RBCC family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: cytoplasm; plasma membrane; nuclear speck; nucleus

Molecular Function: zinc ion binding; ubiquitin-protein ligase activity; ligase activity

Biological Process: apoptosis; protein ubiquitination