Mouse anti-Human TRIM45 Polyclonal Antibody | anti-TRIM45 antibody

TRIM45 (Tripartite Motif-containing Protein 45, RING Finger Protein 99, FLJ13181, RNF99)

Cat. Num. AAA6001850

• Gene Names: TRIM45; RNF99
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: TRIM45; Polyclonal Antibody; TRIM45 (Tripartite Motif-containing Protein 45; RING Finger Protein 99; FLJ13181; RNF99); Anti -TRIM45 (Tripartite Motif-containing Protein 45; anti-TRIM45 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRIM45.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSENRKPLLGFVSKLTSGTALGNSGKTHCPLCLGLFKAPRLLPCLHTVCTTCLEQLEPFSVVDIRGGDSDTSSEGSIFQELKPRSLQSQIGILCPVCDAQVDLPMGGVKALTIDHLAVNDVMLESLRGEGQGLVCDLCNDREVEKRCQTCKANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSRIGKPILCPVHPAEELRLFCEFCDRPVCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVEALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLEDIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVVVERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTASFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYISYTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGGQKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQSAPRSLLRTVAL

• Applicable Applications for anti-TRIM45 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human TRIM45, aa1-562 (AAH34943.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TRIM45 expression in transfected 293T cell line by TRIM45 polyclonal antibody. Lane 1: TRIM45 transfected lysate (61.82kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to TRIM45 on HeLa cell. [antibody concentration 10ug/ml].)

Related Product Information for anti-TRIM45 antibody

TRIM45 belongs to a family of tripartite motif (TRIM) proteins that play important roles in a variety of cellular functions, including cell proliferation, differentiation, development, oncogenesis, and apoptosis. TRIM45 may act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. There are two named isoforms.

Product Categories/Family for anti-TRIM45 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 224548923
• NCBI GeneID: 80263
• NCBI Accession #: NP_001139107.1
• NCBI GenBank Nucleotide #: NM_001145635.1
• UniProt Accession #: Q9H8W5; Q53GN0; Q5T2K4; Q5T2K5; Q8IYV6
• Molecular Weight: 64,359 Da
• NCBI Official Full Name: tripartite motif-containing protein 45 isoform 2
• NCBI Official Synonym Full Names: tripartite motif containing 45
• NCBI Official Symbol: TRIM45
• NCBI Official Synonym Symbols: RNF99
• NCBI Protein Information: tripartite motif-containing protein 45; ring finger protein 99; tripartite motif-containing 45
• UniProt Protein Name: Tripartite motif-containing protein 45
• Protein Family: Tripartite motif-containing protein
• UniProt Gene Name: TRIM45
• UniProt Synonym Gene Names: RNF99
• UniProt Entry Name: TRI45_HUMAN

NCBI Description

This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]

Uniprot Description

TRIM45: May act as a transcriptional repressor in mitogen- activated protein kinase signaling pathway. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: nucleoplasm; intercellular bridge; cytoplasm

Molecular Function: zinc ion binding