Western Blot (WB)
(Western Blot analysis of TRIM45 expression in transfected 293T cell line by TRIM45 polyclonal antibody. Lane 1: TRIM45 transfected lysate (61.82kD). Lane 2: Non-transfected lysate.)
Mouse anti-Human TRIM45 Polyclonal Antibody | anti-TRIM45 antibody
TRIM45 (Tripartite Motif-containing Protein 45, RING Finger Protein 99, FLJ13181, RNF99)
Gene Names
TRIM45; RNF99
Reactivity
Human
Applications
Western Blot, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TRIM45; Polyclonal Antibody; TRIM45 (Tripartite Motif-containing Protein 45; RING Finger Protein 99; FLJ13181; RNF99); Anti -TRIM45 (Tripartite Motif-containing Protein 45; anti-TRIM45 antibody
Host
Mouse
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TRIM45.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Sequence
MSENRKPLLGFVSKLTSGTALGNSGKTHCPLCLGLFKAPRLLPCLHTVCTTCLEQLEPFSVVDIRGGDSDTSSEGSIFQELKPRSLQSQIGILCPVCDAQVDLPMGGVKALTIDHLAVNDVMLESLRGEGQGLVCDLCNDREVEKRCQTCKANLCHFCCQAHRRQKKTTYHTMVDLKDLKGYSRIGKPILCPVHPAEELRLFCEFCDRPVCQDCVVGEHREHPCDFTSNVIHKHGDSVWELLKGTQPHVEALEEALAQIHIINSALQKRVEAVAADVRTFSEGYIKAIEEHRDKLLKQLEDIRAQKENSLQLQKAQLEQLLADMRTGVEFTEHLLTSGSDLEILITKRVVVERLRKLNKVQYSTRPGVNDKIRFCPQEKAGQCRGYEIYDLHRAREKQTASFTLLCKDAAGEIMGRGGDNVQVAVVPKDKKDSPVRTMVQDNKDGTYYISYTPKEPGVYTVWVCIKEQHVQGSPFTVTVRRKHRPHSGVFHCCTFCSSGGQKTARCACGGTMPGGYLGCGHGHKGHPGHPHWSCCGKFNEKSECTWTGGQSAPRSLLRTVAL
Applicable Applications for anti-TRIM45 antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence and Western Blot.
Dilution: Immunofluorescence: 10ug/ml
Dilution: Immunofluorescence: 10ug/ml
Immunogen
Full length human TRIM45, aa1-562 (AAH34943.1).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of TRIM45 expression in transfected 293T cell line by TRIM45 polyclonal antibody. Lane 1: TRIM45 transfected lysate (61.82kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of TRIM45 expression in transfected 293T cell line by TRIM45 polyclonal antibody. Lane 1: TRIM45 transfected lysate (61.82kD). Lane 2: Non-transfected lysate.)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to TRIM45 on HeLa cell. [antibody concentration 10ug/ml].)
Immunofluorescence (IF)
(Immunofluorescence of purified antibody to TRIM45 on HeLa cell. [antibody concentration 10ug/ml].)
Related Product Information for anti-TRIM45 antibody
TRIM45 belongs to a family of tripartite motif (TRIM) proteins that play important roles in a variety of cellular functions, including cell proliferation, differentiation, development, oncogenesis, and apoptosis. TRIM45 may act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway. There are two named isoforms.
Product Categories/Family for anti-TRIM45 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,359 Da
NCBI Official Full Name
tripartite motif-containing protein 45 isoform 2
NCBI Official Synonym Full Names
tripartite motif containing 45
NCBI Official Symbol
TRIM45
NCBI Official Synonym Symbols
RNF99
NCBI Protein Information
tripartite motif-containing protein 45; ring finger protein 99; tripartite motif-containing 45
UniProt Protein Name
Tripartite motif-containing protein 45
Protein Family
UniProt Gene Name
TRIM45
UniProt Synonym Gene Names
RNF99
UniProt Entry Name
TRI45_HUMAN
NCBI Description
This gene encodes a member of the tripartite motif family. The encoded protein may function as a transcriptional repressor of the mitogen-activated protein kinase pathway. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]
Uniprot Description
TRIM45: May act as a transcriptional repressor in mitogen- activated protein kinase signaling pathway. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 1p13.1
Cellular Component: nucleoplasm; intercellular bridge; cytoplasm
Molecular Function: zinc ion binding
Research Articles on TRIM45
Similar Products
Product Notes
The TRIM45 trim45 (Catalog #AAA6001850) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TRIM45 (Tripartite Motif-containing Protein 45, RING Finger Protein 99, FLJ13181, RNF99) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRIM45 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). Suitable for use in Immunofluorescence and Western Blot. Dilution: Immunofluorescence: 10ug/ml. Researchers should empirically determine the suitability of the TRIM45 trim45 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSENRKPLLG FVSKLTSGTA LGNSGKTHCP LCLGLFKAPR LLPCLHTVCT TCLEQLEPFS VVDIRGGDSD TSSEGSIFQE LKPRSLQSQI GILCPVCDAQ VDLPMGGVKA LTIDHLAVND VMLESLRGEG QGLVCDLCND REVEKRCQTC KANLCHFCCQ AHRRQKKTTY HTMVDLKDLK GYSRIGKPIL CPVHPAEELR LFCEFCDRPV CQDCVVGEHR EHPCDFTSNV IHKHGDSVWE LLKGTQPHVE ALEEALAQIH IINSALQKRV EAVAADVRTF SEGYIKAIEE HRDKLLKQLE DIRAQKENSL QLQKAQLEQL LADMRTGVEF TEHLLTSGSD LEILITKRVV VERLRKLNKV QYSTRPGVND KIRFCPQEKA GQCRGYEIYD LHRAREKQTA SFTLLCKDAA GEIMGRGGDN VQVAVVPKDK KDSPVRTMVQ DNKDGTYYIS YTPKEPGVYT VWVCIKEQHV QGSPFTVTVR RKHRPHSGVF HCCTFCSSGG QKTARCACGG TMPGGYLGCG HGHKGHPGHP HWSCCGKFNE KSECTWTGGQ SAPRSLLRTV AL. It is sometimes possible for the material contained within the vial of "TRIM45, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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