Rabbit anti-Human SET7 Polyclonal Antibody | anti-SET7 antibody

SET7, CT (Histone H3-K4 Methyltransferase SETD7, H3-K4-HMTase SETD7, SET Domain-Containing Protein 7, Set9, SET7/9, Lysine N-methyltransferase 7, Histone-lysine N-methyltransferase SETD7, KMT7, KIAA1717) (APC)

Cat. Num. AAA6499177

• Gene Names: SETD7; KMT7; SET7; SET9; SET7/9
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: SET7; Polyclonal Antibody; CT (Histone H3-K4 Methyltransferase SETD7; H3-K4-HMTase SETD7; SET Domain-Containing Protein 7; Set9; SET7/9; Lysine N-methyltransferase 7; Histone-lysine N-methyltransferase SETD7; KMT7; KIAA1717) (APC); anti-SET7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SET7.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SET7 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human SET7 (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-SET7 Pab in mouse brain tissue lysate (35ug/lane). SET7 (arrow) was detected using the purified Pab.)

Product Categories/Family for anti-SET7 antibody

Antibodies; Enzymes; Methyltransferase

References

1. Chuikov, S., et al., Nature 432(7015):353-360 (2004). 2. Wysocka, J., et al., Genes Dev. 17(7):896-911 (2003). 3. Xiao, B., et al., Nature 421(6923):652-656 (2003). 4. Kwon, T., et al., EMBO J. 22(2):292-303 (2003). 5. Nishioka, K., et al., Genes Dev. 16(4):479-489 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 18139549
• NCBI GeneID: 80854
• NCBI Accession #: NP_085151
• NCBI GenBank Nucleotide #: NM_030648.3
• UniProt Accession #: Q8WTS6; Q0VAH3; Q4W5A9; Q9C0E6; B5WWL3
• Molecular Weight: 40,721 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETD7 isoform 1
• NCBI Official Synonym Full Names: SET domain containing lysine methyltransferase 7
• NCBI Official Symbol: SETD7
• NCBI Official Synonym Symbols: KMT7; SET7; SET9; SET7/9
• NCBI Protein Information: histone-lysine N-methyltransferase SETD7
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD7
• Protein Family: SET domain-containing protein
• UniProt Gene Name: SETD7
• UniProt Synonym Gene Names: KIAA1717; KMT7; SET7; SET9; H3-K4-HMTase SETD7
• UniProt Entry Name: SETD7_HUMAN

Uniprot Description

SETD7: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Interacts with IPF1/PDX-1. Widely expressed. Expressed in pancreatic islets. Belongs to the histone-lysine methyltransferase family. SET7 subfamily.

Protein type: Amino Acid Metabolism - lysine degradation; EC 2.1.1.43; Methyltransferase, protein lysine; Methyltransferase

Chromosomal Location of Human Ortholog: 4q28

Cellular Component: nucleoplasm; nucleolus; chromosome

Molecular Function: protein binding; p53 binding; protein-lysine N-methyltransferase activity; histone-lysine N-methyltransferase activity

Biological Process: establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of transcription, DNA-dependent; peptidyl-lysine di-methylation; peptidyl-lysine mono-methylation; chromatin modification; response to DNA damage stimulus