Mouse PNL2 Monoclonal Antibody | anti-PNL2 antibody

PNL2 (Melanoma Associated Antigen) Mouse Monoclonal Antibody

Cat. Num. AAA439689

• Reactivity: Human, Mouse, Dog
• Applications: Immunofluorescence, Immunohistochemistry
• Synonyms: PNL2; Monoclonal Antibody; PNL2 (Melanoma Associated Antigen) Mouse Monoclonal Antibody; Human Melanoma Associated Antigen; anti-PNL2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: PNL2
• Specificity: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87%), as opposed to 76% for anti-HMB45 and 82% for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 379

• Applicable Applications for anti-PNL2 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Melanocyte antigen
• Positive Control: Melanoma
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with PNL2 Monoclonal Antibody.)

References

Rochaix, P et al. PNL2, a New Monoclonal Antibody Directed against a Fixative-Resistant Melanocyte Antigen. Mod Pathol 2003;16(5):481-490. J. A. Ramos-Vara and M. A. Miller. Immunohistochemical Identification of Canine Melanocytic Neoplasms With Antibodies to Melanocytic Antigen PNL2 and Tyrosinase: Comparison With Melan A. Veterinary Pathology OnlineFirst, published on September 21, 2010 as doi:10.1177/0300985810382095

NCBI and Uniprot Product Information

• NCBI GI #: 363411999
• Molecular Weight: 39,348 Da
• NCBI Official Full Name: pectin lyase 2
• UniProt Protein Name: Pectin lyase 2
• UniProt Gene Name: pnl2
• UniProt Entry Name: G9IE21_COLLN