Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Western Blot (WB) (Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)

Rabbit anti-Mouse Trib3 Polyclonal Antibody | anti-Trib3 antibody

Mouse Trib3 Antibody (C-term)

Gene Names
Trib3; Nipk; SINK; Trb3; Ifld2; SKIP3; TRB-3
Reactivity
Mouse
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Trib3; Polyclonal Antibody; Mouse Trib3 Antibody (C-term); Tribbles homolog 3; TRB-3; Neuronal cell death-inducible putative kinase; Nipk; Trb3; anti-Trib3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Trib3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 306-334 amino acids from the C-terminal region of mouse Trib3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
306-334
Sequence Length
354
Applicable Applications for anti-Trib3 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)

Western Blot (WB) (Mouse Trib3 Antibody (C-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Trib3 antibody detected the Trib3 protein (arrow).)
Related Product Information for anti-Trib3 antibody
Trib3 disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Product Categories/Family for anti-Trib3 antibody
References
Morse, E., et al. Am. J. Physiol. Renal Physiol. 299 (5), F965-F972 (2010) :
Dedhia, P.H., et al. Blood 116(8):1321-1328(2010)
Liew, C.W., et al. J. Clin. Invest. 120(8):2876-2888(2010)
Humphrey, R.K., et al. J. Biol. Chem. 285(29):22426-22436(2010)
Liu, J., et al. Am. J. Physiol. Endocrinol. Metab. 298 (3), E565-E576 (2010) :

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
39023
NCBI Official Full Name
tribbles homolog 3
NCBI Official Synonym Full Names
tribbles homolog 3 (Drosophila)
NCBI Official Symbol
Trib3
NCBI Official Synonym Symbols
Nipk; SINK; Trb3; Ifld2; SKIP3; TRB-3
NCBI Protein Information
tribbles homolog 3
UniProt Protein Name
Tribbles homolog 3
Protein Family
UniProt Gene Name
Trib3
UniProt Synonym Gene Names
Nipk; Trb3; TRB-3
UniProt Entry Name
TRIB3_MOUSE

Uniprot Description

TRB3: a Ca(2)/calmodulin-dependent protein kinase of the Trbl family. Induced by the transcription factor NF-kappaB. The encoded protein is a negative regulator of NF-kappaB and can also sensitize cells to TNF- and TRAIL-induced apoptosis. May negatively regulate the cell survival kinase AKT1.

Protein type: Protein kinase, CAMK; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CAMK group; Trbl family

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; enzyme binding; protein kinase inhibitor activity; ubiquitin-protein ligase regulator activity; ubiquitin protein ligase binding; transferase activity, transferring phosphorus-containing groups; transcription corepressor activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: transcription, DNA-dependent; positive regulation of protein binding; apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of fat cell differentiation; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; cellular response to insulin stimulus; regulation of MAP kinase activity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of ubiquitin-protein ligase activity; positive regulation of protein catabolic process; negative regulation of protein kinase activity; negative regulation of transcription, DNA-dependent; negative regulation of fatty acid biosynthetic process

Research Articles on Trib3

Similar Products

Product Notes

The Trib3 trib3 (Catalog #AAA9209520) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 306-334. The Mouse Trib3 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Trib3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Trib3 trib3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Trib3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.