Testing Data
(Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with biotinylated llysine antibody.)
Rabbit Tri-methyl Lysine Polyclonal Antibody
Anti-Tri-methyl Lysine, Biotin
Reactivity
Human, rat, mouse, bovine, pig
Applications
ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
Purity
N-(epsilon) trimethyllysine on Agarose and immunoaffinity adsorbed with N-(epsilon) methylated lysine and N-(epsilon)dimehtylated Lysine on Agarose.
Synonyms
Tri-methyl Lysine; Polyclonal Antibody; Anti-Tri-methyl Lysine; Biotin; Pan N-(epsilon) Tri-MethylLysine Antibody(Biotin Conjugates); anti-Tri-methyl Lysine antibody
Host
Rabbit
Reactivity
Human, rat, mouse, bovine, pig
Clonality
Polyclonal
Specificity
Recognize proteins with trimethylation on lysine residues (N-epsilon)). Not cross-react with acetylated proteins and mono- and dimethylated proteins
Purity/Purification
N-(epsilon) trimethyllysine on Agarose and immunoaffinity adsorbed with N-(epsilon) methylated lysine and N-(epsilon)dimehtylated Lysine on Agarose.
Form/Format
500 umg/mL in PBS, 50% glycerol.
Applicable Applications for anti-Tri-methyl Lysine antibody
ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
Application Notes
ELISA 1:2000, Western blot: 1:2000, Immunoprecipitation: 5 ug/mg protein sample. Immunofluorescent staining: 5 ug/mL or 1:100
Immunogen
Methylated KLH conjugates
Conjugation
NHS-activated d-Biotin.AB/Biotin ratio= approximately 1/10
Preparation and Storage
Store at -20 degree C, stable for up to 12 month.
Testing Data
(Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with biotinylated llysine antibody.)
Testing Data
(Western blot analysis of protein trimethylated from human melanoma cell (MMRU) with biotinylated llysine antibody.)
Related Product Information for anti-Tri-methyl Lysine antibody
This rabbit polyclonal antibody is specific isolated and affinity purified from the anti-serum against chemically methylated protein antigen. The affinity purified trimethylated lysine antibodies are affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The antibodies could be utilized for detection, and quantitation of proteins with N-trimethylated lysine residues.
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Product Notes
The Tri-methyl Lysine (Catalog #AAA330038) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Tri-methyl Lysine, Biotin reacts with Human, rat, mouse, bovine, pig and may cross-react with other species as described in the data sheet. AAA Biotech's Tri-methyl Lysine can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF). ELISA 1:2000, Western blot: 1:2000, Immunoprecipitation: 5 ug/mg protein sample. Immunofluorescent staining: 5 ug/mL or 1:100. Researchers should empirically determine the suitability of the Tri-methyl Lysine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tri-methyl Lysine, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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