Rabbit anti-Human TRH Polyclonal Antibody | anti-TRH antibody

TRH (Prothyroliberin, Thyroliberin, Protirelin, TSH-releasing Factor, TRF, Thyrotropin-releasing Hormone, Thyrotropin-releasing Factor) (HRP)

Cat. Num. AAA6397142

• Gene Names: TRH; TRF; Pro-TRH
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRH; Polyclonal Antibody; TRH (Prothyroliberin; Thyroliberin; Protirelin; TSH-releasing Factor; TRF; Thyrotropin-releasing Hormone; Thyrotropin-releasing Factor) (HRP); anti-TRH antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRH.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1560

• Applicable Applications for anti-TRH antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TRH, aa1-242 (NP_009048.1).
• Immunogen Sequence: MPGPWLLLALALTLNLTGVPGGRAQPEAAQQEAVTAAEHPGLDDFLRQVERLLFLRENIQRLQGDQGEHSASQIFQSDWLSKRQHPGKREEEEEEGVEEEEEEEGGAVGPHKRQHPGRREDEASWSVDVTQHKRQHPGRRSPWLAYAVPKRQHPGRRLADPKAQRSWEEEEEEEEREEDLMPEKRQHPGKRALGGPCGPQGAYGQAGLLLGLLDDLSRSQGAEEKRQHPGRRAAWVREPLEE
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TRH expression in transfected 293T cell line by TRH polyclonal antibody. Lane 1: TRH transfected lysate (27.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-TRH antibody

Thyrotropin-releasing hormone (TRH), also called thyrotropin-releasing factor (TRF), thyroliberin or protirelin, is a tropic, tripeptidal hormone that stimulates the release of TSH (thyroid-stimulating hormone) and prolactin from the anterior pituitary. TRH has been used clinically for the treatment of spinocerebellar degeneration and disturbance of consciousness in humans. TRH is produced by the hypothalamus in medial neurons of the paraventricular nucleus. At the beginning, it is synthesized as a 242aa precursor polypeptide that contains 6 copies of the sequence-Glu-His-Pro-Gly-, flanked by di-basic peptides that are later processed through proteolysis to give the mature TRH molecule. TRH travels across the median eminence to the anterior pituitary gland via the hypophyseal portal system where it stimulates the release of thyroid-stimulating hormone from cells called thyrotropes and excess levels inhibit dopamine which will then stimulate the release of prolactin which in turn decreases GnRH. TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets, as well as in the brain.

Product Categories/Family for anti-TRH antibody

Antibodies; Hormones; Steroids

NCBI and Uniprot Product Information

• NCBI GI #: 1519242950
• NCBI GeneID: 7200
• NCBI Accession #: NM_007117.5
• NCBI GenBank Nucleotide #: NM_007117
• NCBI Official Full Name: Homo sapiens thyrotropin releasing hormone (TRH), mRNA
• NCBI Official Synonym Full Names: thyrotropin releasing hormone
• NCBI Official Symbol: TRH
• NCBI Official Synonym Symbols: TRF; Pro-TRH
• NCBI Protein Information: thyrotropin releasing hormone
• Protein Family: Thyrotropin-releasing hormone

NCBI Description

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]