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Western Blot (WB)
(Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.)
Rabbit DFF40 Polyclonal Antibody | anti-Dffb antibody
DFF40 Antibody
Gene Names
Dffb; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
DFF40 Antibody is affinity chromatography purified via peptide column.
Synonyms
DFF40; Polyclonal Antibody; DFF40 Antibody; CAD; CPAN; 40kDa; Didff; 5730477D02Rik; Cad; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; DNA fragmentation factor; beta subunit; anti-Dffb antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
DFF40 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Sequence Length
344
Applicable Applications for anti-Dffb antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 mug/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL.
Conjugate
Unconjugated
Immunogen
DFF40 antibody was raised against a peptide corresponding to amino acids near the center of murine CAD. The sequence differs from human DFF40 by two amino acids.
Buffer
DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
DFF40 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.)
Western Blot (WB)
(Western blot analysis of DFF40 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with DFF40 antibody (I18) at 1:500 dilution.)
Immunocytochemistry (IHC)
(Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/ml.)
Immunofluorescence (IF)
(Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/mL.Green: DFF40 Antibody (2107)Blue: DAPI staining)
Immunofluorescence (IF)
(Immunofluorescence of DFF40 in K562 cells with DFF40 antibody at 20 μg/mL.Green: DFF40 Antibody (2107)Blue: DAPI staining)
Related Product Information for anti-Dffb antibody
DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40 kDa
NCBI Official Full Name
DNA fragmentation factor subunit beta
NCBI Official Synonym Full Names
DNA fragmentation factor, beta subunit
NCBI Official Symbol
Dffb
NCBI Official Synonym Symbols
CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
NCBI Protein Information
DNA fragmentation factor subunit beta; DFF-40; caspase-activated DNase; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNase inhibited by DNA fragmentation factor; DNA fragmentation factor, 40 kD, beta subunit
UniProt Protein Name
DNA fragmentation factor subunit beta
Protein Family
UniProt Gene Name
Dffb
UniProt Synonym Gene Names
Cad; CAD; Caspase-activated DNase; DFF-40
UniProt Entry Name
DFFB_MOUSE
Uniprot Description
DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Deoxyribonuclease; Apoptosis; EC 3.-.-.-
Cellular Component: nuclear chromatin; cytoplasm; nucleolus; intracellular; nucleus; cytosol
Molecular Function: protein binding; enzyme binding; nuclease activity; DNA binding; hydrolase activity; deoxyribonuclease activity; GPI-anchor transamidase activity
Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation
Research Articles on Dffb
Similar Products
Product Notes
The Dffb dffb (Catalog #AAA150514) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DFF40 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DFF40 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC). DFF40 antibody can be used for detection of DFF40 by Western blot 0.5 mug/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the Dffb dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DFF40, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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