Rabbit TREX1 Polyclonal Antibody | anti-TREX1 antibody

TREX1 Antibody

Cat. Num. AAA150642

• Gene Names: TREX1; CRV; AGS1; DRN3; HERNS
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: TREX1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: TREX1; Polyclonal Antibody; TREX1 Antibody; CRV; AGS1; DRN3; HERNS; Three prime repair exonuclease 1; DNase III; three prime repair exonuclease 1; anti-TREX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TREX1 antibody will not cross-react with the related protein TREX2.
• Purity/Purification: TREX1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 369

• Applicable Applications for anti-TREX1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: TREX1 antibody can be used for detection of TREX1 by Western blot at 0.5 and 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: TREX1 antibody was raised against a 15 amino acid synthetic peptide near the center of human TREX1.
• Buffer: TREX1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: TREX1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of TREX1 in human spleen tissue lysate with TREX1 antibody at (A) 0.5 and (B) 1 μg/mL. )

Immunohistochemistry (IHC)

(Immunohistochemistry of TREX1 in human spleen tissue with TREX1 antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of TREX1 in Human Spleen tissue with TREX1 antibody at 20 μgg/mL.)

Related Product Information for anti-TREX1 antibody

TREX1 Antibody: Trex1 is the major human 3' to 5' exonuclease which is required for checkpoint signaling after DNA damage. It is ubiquitously expressed, binds to single stranded DNA coated with replication protein A that accumulates at sites of DNA damage and recruits the ataxia telangiectasia and Rad3 related protein (ATR), a checkpoint kinase, to sites of DNA damage and replication stress. Trex1 is required for ATR expression. This gene uses two different open reading frames. The upstream ORF encodes proteins which interact with ATR and localize to intranuclear foci induced by DNA damage and are essential components of the DNA damage checkpoint. The downstream ORF encodes proteins with 3' to 5' exonuclease activity and may be a subunit of human DNA polymerase III. Multiple transcript variants encoding different isoforms have been found. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, and Cree encephalitis.

NCBI and Uniprot Product Information

• NCBI GI #: 7705353
• NCBI GeneID: 11277
• NCBI Accession #: NP_057465
• NCBI GenBank Nucleotide #: NM_016381.5
• UniProt Accession #: Q9NSU2; Q8TEU2; Q9BPW1; Q9Y4X2; B2RCN9
• Molecular Weight: 33,212 Da
• NCBI Official Full Name: three-prime repair exonuclease 1 isoform a
• NCBI Official Synonym Full Names: three prime repair exonuclease 1
• NCBI Official Symbol: TREX1
• NCBI Official Synonym Symbols: CRV; AGS1; DRN3; HERNS
• NCBI Protein Information: three-prime repair exonuclease 1; DNase III; deoxyribonuclease III; 3' repair exonuclease 1; 3'-5' exonuclease TREX1
• UniProt Protein Name: Three-prime repair exonuclease 1
• Protein Family: Three-prime repair exonuclease
• UniProt Gene Name: TREX1
• UniProt Entry Name: TREX1_HUMAN

NCBI Description

This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2012]

Uniprot Description

TREX1: the major 3' DNA exonuclease in mammalian cells. Normally associates with the endoplasmic reticulum (ER). Translocates to the nucleus at S phase after DNA damage by gamma-irradiation or hydroxyurea. Trex1-deficient cells show defective G1/S transition, with single-stranded DNA molecules persisting after S phase and accumulating in the ER. Degrades ssDNA. Prevents chronic checkpoint activation and inappropriate immune activation. Mutations cause Aicardi-Goutieres syndrome, an autoimmune disorder. Trex1a(-/-) mice have autoinflammatory responses. The gene for this protein is either identical to or adjacent to that of ATRIP. Some of the mRNAs that encode TREX1 also encode ATRIP in another reading frame. Three alternatively spliced human isoforms have been reported.

Protein type: EC 3.1.11.2; Cell cycle regulation; DNA-binding; DNA repair, damage; Deoxyribonuclease

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: endoplasmic reticulum membrane; nucleolus; nuclear envelope; nucleus; cytosol

Molecular Function: protein homodimerization activity; metal ion binding; double-stranded DNA binding; exodeoxyribonuclease III activity; 3'-5'-exodeoxyribonuclease activity; MutLalpha complex binding; adenyl deoxyribonucleotide binding; 3'-5' exonuclease activity; MutSalpha complex binding; single-stranded DNA binding

Biological Process: regulation of interferon type I production; mismatch repair; positive regulation of interferon type I production; innate immune response; DNA replication; DNA repair; DNA catabolic process, exonucleolytic; DNA metabolic process; DNA recombination

Disease: Chilblain Lupus 1; Vasculopathy, Retinal, With Cerebral Leukodystrophy; Aicardi-goutieres Syndrome 1; Systemic Lupus Erythematosus