Mouse anti-Human GOK / STIM1 Monoclonal Antibody | anti-GOK antibody

Mouse Monoclonal [clone 5A2] (IgG2a,k) to Human GOK / STIM1

Cat. Num. AAA245849

• Gene Names: STIM1; GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: GOK / STIM1; Monoclonal Antibody; Mouse Monoclonal [clone 5A2] (IgG2a; k) to Human GOK / STIM1; Anti-GOK / STIM1 Antibody (clone 5A2) IHC-plus; STIM1; D11S4896E; Stromal interaction molecule 1; GOK; STIM1L; Human GOK; anti-GOK antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 5A2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2. Sourced in Ascites.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 685

• Applicable Applications for anti-GOK antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000); Usage: Western Blot (Tissue lysate) - positive control human liver. Immunofluorescence (10 ug/ml) - positive control HeLa cells. Sandwich ELISA (Recombinant protein).
• Target Species: Human
• Immunogen Description: STIM1 (AAH21300, 24 a.a. ~ 686 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen Type: Recombinant protein
• Immunogen: GOK / STIM1 antibody was raised against sTIM1 (AAH21300, 24 a.a. ~ 686 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.

Immunohistochemistry (IHC)

(Anti-STIM1 antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-STIM1 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to STIM1 on HeLa cell (antibody concentration 10 ug/ml).)

Western Blot (WB)

(STIM1 monoclonal antibody clone 5A2. Western blot of STIM1 expression in human liver.)

ELISA (EIA)

(Detection limit for recombinant GST tagged STIM1 is approximately 0.3 ng/ml as a capture antibody.)

NCBI and Uniprot Product Information

• NCBI GI #: 21070997
• NCBI GeneID: 6786
• NCBI Accession #: NP_003147.2
• NCBI GenBank Nucleotide #: NM_003156.3
• UniProt Accession #: Q13586; Q8N382; E9PQJ4
• Molecular Weight: 62,133 Da
• NCBI Official Full Name: stromal interaction molecule 1 isoform 2
• NCBI Official Synonym Full Names: stromal interaction molecule 1
• NCBI Official Symbol: STIM1
• NCBI Official Synonym Symbols: GOK; TAM; TAM1; IMD10; STRMK; D11S4896E
• NCBI Protein Information: stromal interaction molecule 1
• UniProt Protein Name: Stromal interaction molecule 1
• UniProt Gene Name: STIM1
• UniProt Synonym Gene Names: GOK
• UniProt Entry Name: STIM1_HUMAN

NCBI Description

This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

STIM1: Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit, TMEM142A/ORAI1. Forms homooligomers and heterooligomers with STIM2. Interacts with ORAI1. Interacts with MAPRE1; probably required for targeting to the growing microtubule plus ends. Interacts with EFCAB4B/CRACR2A; the interaction is direct and takes place in absence of Ca(2+). Forms a complex with EFCAB4B/CRACR2A and ORAI1 at low concentration of Ca(2+), the complex dissociates at elevated Ca(2+) concentrations. Interacts with TMEM66/SARAF, promoting a slow inactivation of STIM1-dependent SOCE activity, possibly by facilitating the deoligomerization of STIM1. Ubiquitously expressed in various human primary cells and tumor cell lines.

Protein type: Endoplasmic reticulum; Calcium-binding; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: microtubule; endoplasmic reticulum membrane; endoplasmic reticulum; integral to plasma membrane; integral to endoplasmic reticulum membrane

Molecular Function: identical protein binding; microtubule plus-end binding; protein binding; calcium ion binding

Biological Process: activation of store-operated calcium channel activity; calcium ion transport; detection of calcium ion; regulation of calcium ion transport; blood coagulation

Disease: Myopathy, Tubular Aggregate, 1; Immunodeficiency 10