Western Blot (WB)
(TRAPPC3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TRAPPC3 antibody detected the TRAPPC3 protein (arrow).)
Rabbit anti-Human TRAPPC3 Polyclonal Antibody | anti-TRAPPC3 antibody
TRAPPC3, NT (TRAPPC3, BET3, Trafficking protein particle complex subunit 3, BET3 homolog) (FITC)
Gene Names
TRAPPC3; BET3
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TRAPPC3; Polyclonal Antibody; NT (TRAPPC3; BET3; Trafficking protein particle complex subunit 3; BET3 homolog) (FITC); BET3 homolog; anti-TRAPPC3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-TRAPPC3 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
TRAPPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-54 amino acids from the N-terminal region of human TRAPPC3.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TRAPPC3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TRAPPC3 antibody detected the TRAPPC3 protein (arrow).)
Western Blot (WB)
(TRAPPC3 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the TRAPPC3 antibody detected the TRAPPC3 protein (arrow).)
Flow Cytometry (FC/FACS)
(TRAPPC3 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TRAPPC3 Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TRAPPC3 antibody
TRAPPC3 is a component of the TRAPP complex, which is involved in tethering of transport vesicles to the cis-Golgi membrane (Turnbull et al., 2005 [PubMed 15692564]).[supplied by OMIM].
Product Categories/Family for anti-TRAPPC3 antibody
References
Kummel, D., et al. Proc. Natl. Acad. Sci. U.S.A. 103(34):12701-12706(2006). Kummel, D., et al. J. Mol. Biol. 361(1):22-32(2006). Yu, S., et al. J. Cell Biol. 174(3):359-368(2006). Kim, M.S., et al. Traffic 6(12):1183-1195(2005). Loh, E., et al. J. Cell. Sci. 118 (PT 6), 1209-1222 (2005).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22.4 kDa
NCBI Official Full Name
trafficking protein particle complex subunit 3 isoform 1
NCBI Official Synonym Full Names
trafficking protein particle complex 3
NCBI Official Symbol
TRAPPC3
NCBI Official Synonym Symbols
BET3
NCBI Protein Information
trafficking protein particle complex subunit 3
UniProt Protein Name
Trafficking protein particle complex subunit 3
Protein Family
UniProt Gene Name
TRAPPC3
UniProt Synonym Gene Names
BET3
UniProt Entry Name
TPPC3_HUMAN
Uniprot Description
TRAPPC3: May play a role in vesicular transport from endoplasmic reticulum to Golgi. Belongs to the TRAPP small subunits family. BET3 subfamily.
Protein type: Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 1p34.3
Cellular Component: Golgi apparatus; endoplasmic reticulum
Molecular Function: protein binding
Biological Process: vesicle-mediated transport
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Product Notes
The TRAPPC3 trappc3 (Catalog #AAA6357992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRAPPC3, NT (TRAPPC3, BET3, Trafficking protein particle complex subunit 3, BET3 homolog) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRAPPC3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TRAPPC3 trappc3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TRAPPC3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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