Western Blot (WB)
((0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat Transglutaminase 3 Polyclonal Antibody | anti-TGM3 antibody
Goat anti-Transglutaminase 3 (aa598-610) Antibody
Western Blot: Approx 80kDa band observed in Human Tonsil lysates (calculated MW of 76.6kDa according to NP_003236.3). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Tonsil lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]
Uniprot Description
TGM3: Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold. In hair follicles, involved in cross- linking structural proteins to hardening the inner root sheath. Belongs to the transglutaminase superfamily. Transglutaminase family.
Protein type: EC 2.3.2.13; Membrane protein, peripheral; Transferase
Chromosomal Location of Human Ortholog: 20q11.2
Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane
Molecular Function: calcium ion binding; catalytic activity; protein-glutamine gamma-glutamyltransferase activity; transferase activity, transferring acyl groups
Biological Process: cell envelope organization and biogenesis; hair follicle morphogenesis; keratinocyte differentiation; peptide cross-linking; protein tetramerization
Research Articles on TGM3
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Product Notes
The TGM3 tgm3 (Catalog #AAA422739) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Transglutaminase 3 (aa598-610) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Transglutaminase 3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 32000. Western Blot: Approx 80kDa band observed in Human Tonsil lysates (calculated MW of 76.6kDa according to NP_003236.3). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the TGM3 tgm3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TLEVLNEARV RKP. It is sometimes possible for the material contained within the vial of "Transglutaminase 3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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