TGM3 / Transglutaminase 3 Recombinant Protein | TGM3 recombinant protein

Recombinant Human TGM3 / Transglutaminase 3 Protein (His tag)

Cat. Num. AAA2545637

• Gene Names: TGM3; TGE; UHS2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: TGM3 / Transglutaminase 3; Recombinant Human TGM3 / Transglutaminase 3 Protein (His tag); MGC126249; MGC126250; TGE; TGM3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% gly
• Sequence Length: 693

• Application Notes: The recombinant human TGM3 consists of 710 amino acids and predicts a molecular mass of 78.8 kDa. It migrates as an approximately 70 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for TGM3 recombinant protein

Background: Transglutaminases (TGase) are a family of calcium-dependent acyl-transfer enzymes ubiquitously expressed in mammalian cells and responsible for catalyzing covalent cross-links between proteins or peptides. Transglutaminase 3 (TGM3) is a member of a family of Ca2+-dependent enzymes that catalyze covalent cross-linking reactions between proteins or peptides. TGM3 isoform is widely expressed and is important for epithelial barrier formation. It is a zymogen, requiring proteolysis for activity. Calcium-activated TGM3 can bind, hydrolyze, and is inhibited by GTP, despite lacking structural homology with other GTP binding proteins. TGM3 displays a diffuse cytoplasmic distribution in vitro consistent with its proposed role in the early phase of cornified cell envelope assembly in the cytoplasm. TGM3-driven specific isopeptide bonds between intermediate filaments and KAPs participate to the progressive scaffolding of the hair shaft. Additionally, TGM3 may be a novel prognostic biomarker for esophageal squamous cell carcinoma (ESCC).

Description: A DNA sequence encoding the human TGM3 (Q08188) (Ala 2-Glu 693) was expressed, with a polyhistidine tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 189458821
• NCBI GeneID: 7053
• NCBI Accession #: NP_003236.3
• NCBI GenBank Nucleotide #: NM_003245.3
• UniProt Accession #: Q08188; O95933; Q32ML9; Q32MM0; A8K5N6; B2RCR6; D3DVX1
• Molecular Weight: 76,632 Da
• NCBI Official Full Name: protein-glutamine gamma-glutamyltransferase E
• NCBI Official Synonym Full Names: transglutaminase 3
• NCBI Official Symbol: TGM3
• NCBI Official Synonym Symbols: TGE; UHS2
• NCBI Protein Information: protein-glutamine gamma-glutamyltransferase E
• UniProt Protein Name: Protein-glutamine gamma-glutamyltransferase E
• Protein Family: Protein-glutamine gamma-glutamyltransferase
• UniProt Gene Name: TGM3
• UniProt Synonym Gene Names: TG(E); TGE; TGase E; TGase-3

NCBI Description

Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains activated from a single precursor protein by proteolysis. The encoded protein is involved the later stages of cell envelope formation in the epidermis and hair follicle. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath.