Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Mouse anti-Sheep Hemoglobin Beta (HBb) Monoclonal Antibody | anti-HBb antibody
Monoclonal Antibody to Hemoglobin Beta (HBb)
Reactivity
Sheep
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Hemoglobin Beta (HBb); Monoclonal Antibody; Monoclonal Antibody to Hemoglobin Beta (HBb); anti-HBb antibody
Host
Mouse
Reactivity
Sheep
Clonality
Monoclonal
Isotype
IgG
Clone Number
D4
Specificity
The antibody is a mouse monoclonal antibody raised against HBb. It has been selected for its ability to recognize HBb in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFELRRQAC- MLTAEEKAAV TGFWGKVKVD EVGAEALGRL LVVYPWTQRF FEHFGDLSNA DAVMNNPKVK AHGKKVLDSF SNGMKHLDDL KGTFAQLSEL HCDKLHVDPE NFRLLGNVLV
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFELRRQAC- MLTAEEKAAV TGFWGKVKVD EVGAEALGRL LVVYPWTQRF FEHFGDLSNA DAVMNNPKVK AHGKKVLDSF SNGMKHLDDL KGTFAQLSEL HCDKLHVDPE NFRLLGNVLV
Sequence Length
30
Applicable Applications for anti-HBb antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant HBb (Met1~His145) expressed in E.coli.
Cross Reactivity
Sheep
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,073 Da
NCBI Official Full Name
hemoglobin subunit beta isoform X1
NCBI Official Symbol
LOC101105437
NCBI Protein Information
hemoglobin subunit beta
UniProt Protein Name
Hemoglobin subunit beta
Protein Family
UniProt Gene Name
HBB
Uniprot Description
Involved in oxygen transport from the lung to the various peripheral tissues.
Similar Products
Product Notes
The HBb hbb (Catalog #AAA2012518) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Hemoglobin Beta (HBb) reacts with Sheep and may cross-react with other species as described in the data sheet. AAA Biotech's Hemoglobin Beta (HBb) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the HBb hbb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFELRRQAC- MLTAEEKAAV TGFWGKVKVD EVGAEALGRL LVVYPWTQRF FEHFGDLSNA DAVMNNPKVK AHGKKVLDSF SNGMKHLDDL KGTFAQLSEL HCDKLHVDPE NFRLLGNVLV. It is sometimes possible for the material contained within the vial of "Hemoglobin Beta (HBb), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
