Sheep anti-Human Transducin-Like Enhancer of Split 1 Polyclonal Antibody | anti-TLE3 antibody

Transducin-Like Enhancer of Split 1 (TLE1, E(Sp1) Homolog, Enhancer of Split Groucho-like Protein 1, ESG, ESG1, GRG1, Transducin-like Enhancer Protein 1)

Cat. Num. AAA619381

• Gene Names: TLE3; ESG; ESG3; GRG3; HsT18976
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Transducin-Like Enhancer of Split 1; Polyclonal Antibody; Transducin-Like Enhancer of Split 1 (TLE1; E(Sp1) Homolog; Enhancer of Split Groucho-like Protein 1; ESG; ESG1; GRG1; Transducin-like Enhancer Protein 1); Anti -Transducin-Like Enhancer of Split 1 (TLE1; anti-TLE3 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TLE1. In direct ELISAs, this antibody shows ~10% cross-reactivity with rhTLE3 and rhTLE4 and less than 5% cross-reactivity with rhTLE2.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a lyophillized power in PBS, 5% Trehalose. Reconstitute with sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-TLE3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Dilution: ELISA: 0.5-1ug/ml. The detection limit for rhTLE1 is approximately 2ng/well.; Western Blot: 2ug/ml
• Immunogen: E. coli-derived rhTLE1 (aa 11-200)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TLE3 antibody

TLE1 (Transducin-Like Enhancer of Split 1; also ESG1 and GRG1) is a member of the WD repeat Groucho/TLE family of transcriptional repressors. It is widely expressed, and is known to antagonize NFkB and TCF (Wnt)-mediated signaling. TLE1 binds to other family members as a heterooligomer, or to itself as a homooligomer. While it possesses no intrinsic DNA-binding activity, it does modulate the activity of bound transcription factors. Human TLE1 is 770 amino acids (aa) in length. It contains a Gln-rich region that mediates oligomerization (aa1-131), a CCN domain that contains an NLS and CDC2 kinase site (aa200-268), and six WD repeats that mediate protein-protein interaction (aa470-767). Phosphorylation on Ser will generate 90-93kD and 116kD forms in SDS-Page. Potential splice variants exist. There are alternative start sites at Met57 and Met326, a 10aa insertion after Gly124, and three distinct eight aa substitutions for aa 193-770, 193-199 and 445-770. Over aa11-200, human TLE1 shares 98% aa identity with mouse TLE1.

Product Categories/Family for anti-TLE3 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 46391800
• NCBI GeneID: 7090
• UniProt Accession #: Q04726; Q8IVV6; Q8WVR2; Q9HCM5; E9PD64
• Molecular Weight: 83,417 Da
• NCBI Official Full Name: transducin-like enhancer of split 3 splice variant 1
• NCBI Official Synonym Full Names: transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
• NCBI Official Symbol: TLE3
• NCBI Official Synonym Symbols: ESG; ESG3; GRG3; HsT18976
• NCBI Protein Information: transducin-like enhancer protein 3; enhancer of split groucho 3; enhancer of split groucho-like protein 3; transducin-like enhancer of split 3, homolog of Drosophila E(sp1)
• UniProt Protein Name: Transducin-like enhancer protein 3
• Protein Family: Transducin-like enhancer protein
• UniProt Gene Name: TLE3
• UniProt Synonym Gene Names: KIAA1547; ESG3
• UniProt Entry Name: TLE3_HUMAN

NCBI Description

This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]

Uniprot Description

TLE3: transcriptional corepressor that binds to a number of transcription factors. Belongs to the WD-repeat Groucho/TLE family. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Four alternatively spliced isoforms have been described.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: Notch signaling pathway; organ morphogenesis; Wnt receptor signaling pathway; transcription, DNA-dependent; regulation of transcription, DNA-dependent; signal transduction