Western Blot (WB)
(Western Blot analysis of HEK-293 cells using TLE3 Monoclonal Antibody at dilution of 1:1000)
Mouse anti-Human TLE3 Monoclonal Antibody | anti-TLE3 antibody
TLE3 Monoclonal Antibody
Gene Names
TLE3; ESG; ESG3; GRG3; HsT18976
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Protein G purification
Synonyms
TLE3; Monoclonal Antibody; TLE3 Monoclonal Antibody; E(sp1) homolog Drosophila; Enhancer of split groucho 3; Enhancer of split groucho-like protein 3; ESG 3; ESG; ESG3; FLJ39460; GRG 3; GRG3; HsT18976; KIAA1547; TLE 3; Tle3; Transducin like enhancer of split 3; Transducin like enhancer of split 3 homolog of Drosophila E(sp1); Transducin like enhancer protein 3; transducin-like enhancer of split 3 (E(sp1) homolog; Drosophila); Transducin-like enhancer protein 3; anti-TLE3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
654
Purity/Purification
Protein G purification
Concentration
1mg/mL (varies by lot)
Sequence Length
769
Applicable Applications for anti-TLE3 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IHC: 1:20-1:200
Immunogen
Fusion protein of TLE3
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using TLE3 Monoclonal Antibody at dilution of 1:1000)
Western Blot (WB)
(Western Blot analysis of HEK-293 cells using TLE3 Monoclonal Antibody at dilution of 1:1000)
Related Product Information for anti-TLE3 antibody
TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane therapy.
Product Categories/Family for anti-TLE3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Calculated: 83kDa
Observed: 90kDa
Observed: 90kDa
NCBI Official Full Name
transducin-like enhancer protein 3 isoform b
NCBI Official Synonym Full Names
transducin like enhancer of split 3
NCBI Official Symbol
TLE3
NCBI Official Synonym Symbols
ESG; ESG3; GRG3; HsT18976
NCBI Protein Information
transducin-like enhancer protein 3
UniProt Protein Name
Transducin-like enhancer protein 3
Protein Family
UniProt Gene Name
TLE3
UniProt Synonym Gene Names
KIAA1547; ESG3
NCBI Description
This gene encodes a transcriptional co-repressor protein that belongs to the transducin-like enhancer family of proteins. The members of this family function in the Notch signaling pathway that regulates determination of cell fate during development. Expression of this gene has been associated with a favorable outcome to chemotherapy with taxanes for ovarian carcinoma. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Sep 2013]
Uniprot Description
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES ().
Research Articles on TLE3
Similar Products
Product Notes
The TLE3 tle3 (Catalog #AAA2542090) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TLE3 Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TLE3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200. Researchers should empirically determine the suitability of the TLE3 tle3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TLE3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
