Western Blot (WB)
(Lanes:Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline inducedLane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-inducedLane 3: 10ug siRNA scrambled-MDA-MB-231 cellsLane 4: siRNA Tradd-MDA-MB-231 cellsPrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:2000Gene Name:TRADDSubmitted by:Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer ResearchThere is BioGPS gene expression data showing that TRADD is expressed in HEK293T)
Rabbit TRADD Polyclonal Antibody | anti-TRADD antibody
TRADD antibody - middle region
Western Blot (WB)
(Lanes:Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline inducedLane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-inducedLane 3: 10ug siRNA scrambled-MDA-MB-231 cellsLane 4: siRNA Tradd-MDA-MB-231 cellsPrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:2000Gene Name:TRADDSubmitted by:Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer ResearchThere is BioGPS gene expression data showing that TRADD is expressed in HEK293T)
Western Blot (WB)
(Lanes:Lane 1: 10ug Tradd-HA-Strep-stable expression 293TREXFlpIn cells-Doxycycline inducedLane 2: 10ug ITradd-HA-Strep-stable expression 293TREXFlpIn cells-non-inducedLane 3: 10ug siRNA scrambled-MDA-MB-231 cellsLane 4: siRNA Tradd-MDA-MB-231 cellsPrimary Antibody Dilution:1:1000Secondary Antibody:Anti-rabbit HRPSecondary Antibody Dilution:1:2000Gene Name:TRADDSubmitted by:Dr. Tencho Tenev, The Breakthrough Breast Cancer Research Centre, Institute of Cancer ResearchThere is BioGPS gene expression data showing that TRADD is expressed in HEK293T)
Target Description: TRADD is the adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]
Uniprot Description
TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.
Protein type: Adaptor/scaffold; Apoptosis
Chromosomal Location of Human Ortholog: 16q22
Cellular Component: cytoskeleton; cytoplasm; nucleus; cytosol; receptor complex; lipid raft
Molecular Function: identical protein binding; signal transducer activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; kinase binding; molecular adaptor activity
Biological Process: caspase activation; positive regulation of hair follicle development; positive regulation of I-kappaB kinase/NF-kappaB cascade; induction of apoptosis via death domain receptors; tumor necrosis factor-mediated signaling pathway; protein heterooligomerization; apoptosis; positive regulation of apoptosis; signal transduction; activation of NF-kappaB transcription factor
Research Articles on TRADD
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Product Notes
The TRADD tradd (Catalog #AAA3224341) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRADD antibody - middle region reacts with Dog, Horse, Human and may cross-react with other species as described in the data sheet. AAA Biotech's TRADD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TRADD tradd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: YEQAFQLLRR FVQAEGRRAT LQRLVEALEE NELTSLAEDL LGLTDPNGGL. It is sometimes possible for the material contained within the vial of "TRADD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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