Rabbit anti-Human, Mouse TRADD Polyclonal Antibody | anti-TRADD antibody

Rabbit Polyclonal (IgG) to Human TRADD

Cat. Num. AAA243009

• Gene Names: TRADD; Hs.89862
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TRADD; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human TRADD; Anti-TRADD Antibody (aa251-300) IHC-plus; Hs.89862; Human TRADD; anti-TRADD antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TRADD Antibody detects endogenous levels of total TRADD protein.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 312

• Applicable Applications for anti-TRADD antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TRADD (aa251-300).
• Immunogen: TRADD antibody was raised against synthetic peptide from human TRADD (aa251-300).
• Antigen Modification: aa251-300
• Preparation and Storage: Store at -20 degree C for up to one year.

Immunohistochemistry (IHC)

(Anti-TRADD antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Western Blot (WB)

(Western blot of extracts from COS7 cells, using TRADD Antibody. The lane on the right is treated with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 13378137
• NCBI GeneID: 8717
• NCBI Accession #: NP_003780.1
• NCBI GenBank Nucleotide #: NM_003789.3
• UniProt Accession #: Q15628; Q52NZ1; B2RDS3; B3KQZ9
• Molecular Weight: 27,904 Da
• NCBI Official Full Name: tumor necrosis factor receptor type 1-associated DEATH domain protein
• NCBI Official Synonym Full Names: TNFRSF1A-associated via death domain
• NCBI Official Symbol: TRADD
• NCBI Official Synonym Symbols: Hs.89862
• NCBI Protein Information: tumor necrosis factor receptor type 1-associated DEATH domain protein; TNFR1-associated death domain protein; tumor necrosis factor receptor-1-associated protein; tumor necrosis factor receptor type 1 associated death domain protein
• UniProt Protein Name: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• Protein Family: Tumor necrosis factor receptor type 1-associated DEATH domain protein
• UniProt Gene Name: TRADD
• UniProt Synonym Gene Names: TNFR1-associated DEATH domain protein
• UniProt Entry Name: TRADD_HUMAN

NCBI Description

The protein encoded by this gene is a death domain containing adaptor molecule that interacts with TNFRSF1A/TNFR1 and mediates programmed cell death signaling and NF-kappaB activation. This protein binds adaptor protein TRAF2, reduces the recruitment of inhibitor-of-apoptosis proteins (IAPs) by TRAF2, and thus suppresses TRAF2 mediated apoptosis. This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TRADD: Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B. Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP. Found in all examined tissues.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoskeleton; cytoplasm; cytosol; nucleus; receptor complex; lipid raft

Molecular Function: identical protein binding; protein binding; signal transducer activity; protein complex binding; kinase binding; tumor necrosis factor receptor binding; molecular adaptor activity

Biological Process: positive regulation of hair follicle development; caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; tumor necrosis factor-mediated signaling pathway; induction of apoptosis via death domain receptors; positive regulation of apoptosis; apoptosis; protein heterooligomerization; signal transduction; activation of NF-kappaB transcription factor