Rabbit anti-Human RPL3 Polyclonal Antibody | anti-RPL3 antibody

RPL3, ID (RPL3, 60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B)

Cat. Num. AAA6007760

• Gene Names: RPL3; L3; ASC-1; TARBP-B
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RPL3; Polyclonal Antibody; ID (RPL3; 60S ribosomal protein L3; HIV-1 TAR RNA-binding protein B); Anti -RPL3; anti-RPL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-RPL3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: RPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 223-251 amino acids from the Central region of human RPL3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RPL3 Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the RPL3 antibody detected the RPL3 protein (arrow).)

Immunohistochemistry (IHC)

(RPL3 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-RPL3 antibody

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Product Categories/Family for anti-RPL3 antibody

Antibodies; Abs to Infectious Disease HIV

NCBI and Uniprot Product Information

• NCBI GI #: 47678663
• NCBI GeneID: 6122
• UniProt Accession #: P39023; Q15548; Q5I0G0; B2RDV9
• Molecular Weight: 46,109 Da
• NCBI Official Full Name: RPL3
• NCBI Official Synonym Full Names: ribosomal protein L3
• NCBI Official Symbol: RPL3
• NCBI Official Synonym Symbols: L3; ASC-1; TARBP-B
• NCBI Protein Information: 60S ribosomal protein L3; hypothetical protein; HIV-1 TAR RNA-binding protein B; ribosomal protein L3, isoform a
• UniProt Protein Name: 60S ribosomal protein L3
• Protein Family: Ribosomal protein
• UniProt Gene Name: RPL3
• UniProt Synonym Gene Names: TARBP-B
• UniProt Entry Name: RL3_HUMAN

NCBI Description

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

Uniprot Description

RPL3: The L3 protein is a component of the large subunit of cytoplasmic ribosomes. Belongs to the ribosomal protein L3P family.

Protein type: Nucleolus; Ribosomal; Translation

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: nucleoplasm; focal adhesion; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: structural constituent of ribosome; RNA binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; viral transcription; gene expression; viral infectious cycle; translational termination