anti-Human TPO [288-315] IgG fraction Polyclonal Antibody
TPO [288-315] IgG fraction
Reactivity
Human
Applications
Immunoblot, Western Blot, Western Blot
Synonyms
TPO [288-315] IgG fraction; Polyclonal Antibody; Thrombopoietin; TPO (288-315); IgG Fraction of Rabbit Anti-human Thrombopoietin Sera; anti-TPO [288-315] IgG fraction antibody
Reactivity
Human
Clonality
Polyclonal
Specificity
Polypeptide ::: % Cross Reactivity
Thrombopoietin Human (299-332) ::: 100
Thrombopoietin Human recombinant ::: ~90
Thrombopoietin Human (299-332) ::: 100
Thrombopoietin Human recombinant ::: ~90
Form/Format
Antiserum Specificity
Applicable Applications for anti-TPO [288-315] IgG fraction antibody
Immunocytochemical Staining, Western Immunoblot, Western Blot
Application Notes
Immunocytochemistry: The pooled antisera have been found to stain fixed cells know to express human TPO using ABC techniques at a 1:500 dilution.
Western Blot: Western immunoblots using tissue homogenates have been successful at a 1:800 dilution and yield a single band at 59 kDa
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum using TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the antibody after preparing a 1:800 dilution (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash for 5 minutes x 4 in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Western Blot: Western immunoblots using tissue homogenates have been successful at a 1:800 dilution and yield a single band at 59 kDa
Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum using TBS/Tween-20 buffer.
2. Wash x 2 with TBS/Tween-20.
3. Apply the antibody after preparing a 1:800 dilution (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours.
4. Wash x 3 with TBS/Tween-20.
5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.
6. Wash for 5 minutes x 4 in TBS/Tween-20.
7. Develop color using the enhanced DAB reaction.
Related Product Information for anti-TPO [288-315] IgG fraction antibody
These pooled antisera were raised in rabbits which were immunized with a peptide analogue of human thrombopoietin (TPO residues #288-315) attached onto a carrier protein. The antisera have been shown to be specific for TPO. have been characterized by western blotting. ELISA. and cell staining techniques. and are suitable for immunocytochemical and western immunoblotting detection of this hormone. Dilute the lyophilized antibody with 0.1 ml of 10 mg/ml BSA in PBS for the equivalent of whole antiserum. or with additional buffer for more dilute antibody.
Product Categories/Family for anti-TPO [288-315] IgG fraction antibody
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Product Notes
The TPO [288-315] IgG fraction (Catalog #AAA352279) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TPO [288-315] IgG fraction reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TPO [288-315] IgG fraction can be used in a range of immunoassay formats including, but not limited to, Immunocytochemical Staining, Western Immunoblot, Western Blot. Immunocytochemistry: The pooled antisera have been found to stain fixed cells know to express human TPO using ABC techniques at a 1:500 dilution. Western Blot: Western immunoblots using tissue homogenates have been successful at a 1:800 dilution and yield a single band at 59 kDa Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum using TBS/Tween-20 buffer. 2. Wash x 2 with TBS/Tween-20. 3. Apply the antibody after preparing a 1:800 dilution (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20. Let the primary antibody bind for 2-4 hours. 4. Wash x 3 with TBS/Tween-20. 5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques. 6. Wash for 5 minutes x 4 in TBS/Tween-20. 7. Develop color using the enhanced DAB reaction. Researchers should empirically determine the suitability of the TPO [288-315] IgG fraction for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TPO [288-315] IgG fraction, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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