Rabbit anti-Human TPC6A Polyclonal Antibody | anti-TPC6A antibody

TPC6A, NT (TRAPPC6A, Trafficking protein particle complex subunit 6A) (Biotin)

Cat. Num. AAA6357595

• Gene Names: TRAPPC6A; TRS33
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TPC6A; Polyclonal Antibody; NT (TRAPPC6A; Trafficking protein particle complex subunit 6A) (Biotin); TRAPPC6A; Trafficking protein particle complex subunit 6A; anti-TPC6A antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TPC6A antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TPC6A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-72 amino acids from the N-terminal region of human TPC6A.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TPC6A Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the TPC6A antibody detected the TPC6A protein (arrow).)

Immunohistochemistry (IHC)

(TPC6A Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TPC6A Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TPC6A Antibody (N-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TPC6A antibody

TRAPPC6A is a subunit of the TRAPP I and II complexes.

Product Categories/Family for anti-TPC6A antibody

Antibodies; Proteins

References

Lowe, J.K., et al. PLoS Genet. 5 (2), E1000365 (2009). Gwynn, B., et al. Genomics 88(2):196-203(2006). Kim, M.S., et al. Traffic 6(12):1183-1195(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 400153783
• NCBI GeneID: 79090
• NCBI Accession #: NP_001257820.1
• NCBI GenBank Nucleotide #: NM_001270891.1
• UniProt Accession #: O75865; Q9BQ45; Q9P092
• Molecular Weight: 17,605 Da
• NCBI Official Full Name: trafficking protein particle complex subunit 6A isoform 2
• NCBI Official Synonym Full Names: trafficking protein particle complex 6A
• NCBI Official Symbol: TRAPPC6A
• NCBI Official Synonym Symbols: TRS33
• NCBI Protein Information: trafficking protein particle complex subunit 6A; TRAPP complex subunit 6A
• UniProt Protein Name: Trafficking protein particle complex subunit 6A
• UniProt Gene Name: TRAPPC6A
• UniProt Synonym Gene Names: TRAPP complex subunit 6A
• UniProt Entry Name: TPC6A_HUMAN

NCBI Description

This gene encodes a component of the trafficking protein particle complex, which tethers transport vesicles to the cis-Golgi membrane. Loss of expression of the related gene in mouse affects coat and eye pigmentation, suggesting that the encoded protein may be involved in melanosome biogenesis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

TRAPPC6A: May play a role in vesicular transport during the biogenesis of melanosomes. Belongs to the TRAPP small subunits family. BET3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: Golgi apparatus; endoplasmic reticulum

Molecular Function: protein binding

Biological Process: vesicle-mediated transport