Insulin Like Growth Factor 1 (IGF1) Recombinant Protein | IGF1 recombinant protein

Recombinant Insulin Like Growth Factor 1 (IGF1)

Cat. Num. AAA2012247

• Gene Names: IGF1; IGF-I; Npt2B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Insulin Like Growth Factor 1 (IGF1); Recombinant Insulin Like Growth Factor 1 (IGF1); IGF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with a N-terminal GST-tag, its sequence is listed below.; MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD G-GPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
• Sequence Length: 130

• Applicable Applications for IGF1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Gly49~Ala118 (Accession # P16545) with a N-terminal GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47523588
• NCBI GeneID: 397491
• NCBI Accession #: NP_999421.1
• NCBI GenBank Nucleotide #: NM_214256.1
• UniProt Accession #: P16545
• Molecular Weight: 28.4kDa
• NCBI Official Full Name: insulin-like growth factor I
• NCBI Official Symbol: IGF1
• NCBI Official Synonym Symbols: IGF-I; Npt2B
• NCBI Protein Information: insulin-like growth factor I; somatomedin C
• UniProt Protein Name: Insulin-like growth factor I
• Protein Family: Insulin-like growth factor
• UniProt Gene Name: IGF1
• UniProt Synonym Gene Names: IGF-I
• UniProt Entry Name: IGF1_PIG

Uniprot Description

Function: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake

By similarity.

Subcellular location: Secreted.

Sequence similarities: Belongs to the insulin family.

Sequence caution: The sequence AAA31043.1 differs from that shown. Reason: Erroneous initiation.