Rabbit anti-Human, Mouse TP53RK Polyclonal Antibody | anti-TP53RK antibody
TP53RK Antibody
Gene Names
TP53RK; PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
TP53RK; Polyclonal Antibody; TP53RK Antibody; C20orf64; Nori 2; Nori 2p; Nori-2; p53 related protein kinase; p53-related protein kinase; PRPK_HUMAN; TP53 regulating kinase; TP53-regulating kinase; anti-TP53RK antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
TP53RK Antibody detects endogenous levels of TP53RK.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TP53RK antibody
ELISA (EIA)(peptide)
Application Notes
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human TP53RK.
Conjugation
Unconjugated
Similarity
This protein is considered an atypical serine/threonine kinase, because it lacks the conventional structural elements necessary for the substrate recognition as well as a lysine residue that in all other serine/threonine kinases participates in the catalytic event. TP53RK has protein kinase activity in vitro, but in the context of the EKC/KEOPS complex, the catalytic subunit OSGEP switches the activity of TP53RK from kinase into ATPase (By similarity). Belongs to the protein kinase superfamily. BUD32 family.
Subunit Structure
Component of the EKC/KEOPS complex composed of at least GON7, TP53RK, TPRKB, OSGEP and LAGE3; the whole complex dimerizes.
Tissue Specificity
Highly expressed in testis. Weakly expressed in heart kidney and spleen.
Function
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,160 Da
NCBI Official Full Name
TP53-regulating kinase
NCBI Official Synonym Full Names
TP53 regulating kinase
NCBI Official Symbol
TP53RK
NCBI Official Synonym Symbols
PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
NCBI Protein Information
TP53-regulating kinase; p53-related protein kinase; EKC/KEOPS complex subunit TP53RK; atypical serine/threonine protein kinase TP53RK
UniProt Protein Name
TP53-regulating kinase
Protein Family
UniProt Gene Name
TP53RK
UniProt Synonym Gene Names
C20orf64; PRPK
UniProt Entry Name
PRPK_HUMAN
Uniprot Description
PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Other group; Bud32 family
Chromosomal Location of Human Ortholog: 20q13.2
Cellular Component: membrane; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; hydrolase activity; p53 binding; ATP binding
Biological Process: tRNA processing; protein amino acid phosphorylation
Research Articles on TP53RK
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Product Notes
The TP53RK tp53rk (Catalog #AAA9629833) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TP53RK Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TP53RK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA)(peptide). ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the TP53RK tp53rk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TP53RK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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