Immunohistochemistry (IHC)
(Anti-TP53RK antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Mouse anti-Human PRPK / TP53RK Monoclonal Antibody | anti-PRPK antibody
Mouse Monoclonal [clone 4B9-1H3] (IgG1,k) to Human PRPK / TP53RK
Gene Names
TP53RK; PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Protein A Purified
Synonyms
PRPK / TP53RK; Monoclonal Antibody; Mouse Monoclonal [clone 4B9-1H3] (IgG1; k) to Human PRPK / TP53RK; Anti-PRPK / TP53RK Antibody (clone 4B9-1H3) IHC-plus; TP53RK; C20orf64; BUD32; DJ101A2.2; DJ101A2; Nori-2p; p53-related protein kinase; PRPK; TP53-regulating kinase; Nori-2; TP53 regulating kinase; Human PRPK; anti-PRPK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
4B9-1H3
Purity/Purification
Protein A Purified
Form/Format
PBS, pH 7.2
Sequence Length
253
Applicable Applications for anti-PRPK antibody
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Application Notes
ELISA, IHC-P (10 ug/ml), WB
Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).
Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein).
Target Species
Human
Immunogen Description
TP53RK (AAH09727, 1 a.a. ~ 254 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Immunogen Type
Recombinant protein
Immunogen
PRPK / TP53RK antibody was raised against tP53RK (AAH09727, 1 a.a. ~ 254 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Anti-TP53RK antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC)
(Anti-TP53RK antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Western Blot (WB)
(TP53RK monoclonal antibody, clone 4B9-1H3 Western blot of TP53RK expression in Jurkat.)
Western Blot (WB)
(TP53RK monoclonal antibody, clone 4B9-1H3 Western blot of TP53RK expression in Jurkat.)
ELISA (EIA)
(Detection limit for recombinant GST tagged TP53RK is approximately 1 ng/ml as a capture antibody.)
ELISA (EIA)
(Detection limit for recombinant GST tagged TP53RK is approximately 1 ng/ml as a capture antibody.)
Product Categories/Family for anti-PRPK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,160 Da
NCBI Official Full Name
TP53-regulating kinase
NCBI Official Synonym Full Names
TP53 regulating kinase
NCBI Official Symbol
TP53RK
NCBI Official Synonym Symbols
PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
NCBI Protein Information
TP53-regulating kinase; p53-related protein kinase; EKC/KEOPS complex subunit TP53RK; atypical serine/threonine protein kinase TP53RK
UniProt Protein Name
TP53-regulating kinase
UniProt Gene Name
TP53RK
UniProt Synonym Gene Names
C20orf64; PRPK
UniProt Entry Name
PRPK_HUMAN
Uniprot Description
PRPK: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Belongs to the protein kinase superfamily.
Protein type: Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Other group; Bud32 family
Chromosomal Location of Human Ortholog: 20q13.2
Cellular Component: membrane; nucleus; cytosol
Molecular Function: protein serine/threonine kinase activity; protein binding; hydrolase activity; p53 binding; ATP binding
Biological Process: tRNA processing; protein amino acid phosphorylation
Research Articles on PRPK
Similar Products
Product Notes
The PRPK tp53rk (Catalog #AAA246033) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 4B9-1H3] (IgG1,k) to Human PRPK / TP53RK reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PRPK / TP53RK can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA). ELISA, IHC-P (10 ug/ml), WB Usage: Western Blot (Cell lysate) - positive control Jurkat. Western Blot (Transfected lysate) - positive control transfected 293T cell line. Sandwich ELISA (Recombinant protein). Researchers should empirically determine the suitability of the PRPK tp53rk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRPK / TP53RK, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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