SDS-PAGE
TP53RK recombinant protein
TP53RK recombinant protein
Gene Names
TP53RK; PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
Applications
Western Blot
Synonyms
TP53RK; TP53RK recombinant protein; BUD32; C20orf64; dJ101A2; Nori-2; Nori-2p
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
3384
Applicable Applications for TP53RK recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for TP53RK recombinant protein
Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. TP53RK belongs to a small subfamily of atypical protein kinases. TP53RK is expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes and lower expression of TP53RK is found in heart, kidney, and spleen (1). PRPK interacts with CGI-121 and co-precipitation of p53 with PRPK is inhibited by adding recombinant CGI-121 in vitro. CGI-121 may act as an inhibitor of the PRPK-p53 interaction (2). TP53RK is activated by another kinase, Akt/PKB, which phosphorylates TP53RK at Ser250.
Scientific Background: TP53RK or TP53 regulating kinase and also called PRPK (p53-related protein kinase) is the human homologue of yeast Bud32. TP53RK belongs to a small subfamily of atypical protein kinases. TP53RK is expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes and lower expression of TP53RK is found in heart, kidney, and spleen (1). PRPK interacts with CGI-121 and co-precipitation of p53 with PRPK is inhibited by adding recombinant CGI-121 in vitro. CGI-121 may act as an inhibitor of the PRPK-p53 interaction (2). TP53RK is activated by another kinase, Akt/PKB, which phosphorylates TP53RK at Ser250.
Product Categories/Family for TP53RK recombinant protein
References
1. Abe, Y.et.al: Cloning and characterization of a p53-related protein kinase expressed in interleukin-2-activated cytotoxic T-cells, epithelial tumor cell lines, and the testes. J. Biol. Chem. 276: 44003-44011, 2001.
2. Miyoshi, A.et.al: Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. Biochem. Biophys. Res. Commun. 303: 399-405, 2003.
2. Miyoshi, A.et.al: Identification of CGI-121, a novel PRPK (p53-related protein kinase)-binding protein. Biochem. Biophys. Res. Commun. 303: 399-405, 2003.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~55 kDa
NCBI Official Full Name
Homo sapiens TP53 regulating kinase (TP53RK), mRNA
NCBI Official Synonym Full Names
TP53 regulating kinase
NCBI Official Symbol
TP53RK
NCBI Official Synonym Symbols
PRPK; BUD32; Nori-2; Nori-2p; dJ101A2; C20orf64
NCBI Protein Information
TP53-regulating kinase; p53-related protein kinase
UniProt Protein Name
TP53-regulating kinase
Protein Family
UniProt Gene Name
TP53RK
UniProt Synonym Gene Names
C20orf64; PRPK
UniProt Entry Name
PRPK_HUMAN
Uniprot Description
Function: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. Ref.1
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Subunit structure: Interacts with TPRKB. Ref.7
Subcellular location: Nucleus.
Tissue specificity: Highly expressed in testis. Weakly expressed in heart kidney and spleen.
Sequence similarities: Belongs to the protein kinase superfamily.Contains 1 protein kinase domain.
Research Articles on TP53RK
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Product Notes
The TP53RK tp53rk (Catalog #AAA515194) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human TP53RK was expressed by baculovirus in Sf9 insect cells. AAA Biotech's TP53RK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the TP53RK tp53rk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TP53RK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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