Rabbit anti-Human TP53INP1 Polyclonal Antibody | anti-TP53INP1 antibody

TP53INP1, NT (TP53INP1, P53DINP1, SIP, Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1) (AP)

Cat. Num. AAA6357516

• Gene Names: TP53INP1; SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TP53INP1; Polyclonal Antibody; NT (TP53INP1; P53DINP1; SIP; Tumor protein p53-inducible nuclear protein 1; Stress-induced protein; p53-dependent damage-inducible nuclear protein 1) (AP); p53-dependent damage-inducible nuclear protein 1; anti-TP53INP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TP53INP1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-TP53INP1 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 36-64 in the N-terminal region of human TP53INP1, UniProt Accession #Q96A56.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in SK-BR-3 cell line lysates (35ug/lane) usingMBS6010767. This demonstrates that MBS6010767 detected the TP53INP1 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6010767 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6010767 for immunohistochemistry.)

Product Categories/Family for anti-TP53INP1 antibody

Antibodies; Cancer Markers

References

1. Voight, B.F., et al. Nat. Genet. 42(7):579-589(2010). 2. Yeung, M.L., et al. Cancer Res. 68(21):8976-8985(2008). 3. Daniele, B. J. Clin. Gastroenterol. 42(4):336-337(2008). 4. Sawaya, M., et al. J. Clin. Gastroenterol. 42(4):351-355(2008). 5. Bernardo, M.V., et al. Biochem. Biophys. Res. Commun. 359(2):317-322(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 209180469
• NCBI GeneID: 94241
• NCBI Accession #: NP_001129205.1; NP_150601.1
• NCBI GenBank Nucleotide #: NM_001135733.1
• UniProt Accession #: Q96A56; Q969R9; B2RCE5
• Molecular Weight: 27,366 Da
• NCBI Official Full Name: tumor protein p53-inducible nuclear protein 1 isoform b
• NCBI Official Synonym Full Names: tumor protein p53 inducible nuclear protein 1
• NCBI Official Symbol: TP53INP1
• NCBI Official Synonym Symbols: SIP; Teap; p53DINP1; TP53DINP1; TP53INP1A; TP53INP1B
• NCBI Protein Information: tumor protein p53-inducible nuclear protein 1; p53-inducible p53DINP1; stress-induced protein; p53-dependent damage-inducible nuclear protein 1
• UniProt Protein Name: Tumor protein p53-inducible nuclear protein 1
• Protein Family: Tumor protein p53-inducible nuclear protein
• UniProt Gene Name: TP53INP1
• UniProt Synonym Gene Names: P53DINP1; SIP; p53DINP1
• UniProt Entry Name: T53I1_HUMAN

Uniprot Description

TP53INP1: Antiproliferative and proapoptotic protein involved in cell stress response which acts as a dual regulator of transcription and autophagy. Acts as a positive regulator of autophagy. In response to cellular stress or activation of autophagy, relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C and regulates autophagy. Acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. Possesses both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. Positively regulates p53/TP53 and p73/TP73 and stimulates their capacity to induce apoptosis and regulate cell cycle. In response to double-strand DNA breaks, promotes p53/TP53 phosphorylation on 'Ser-46' and subsequent apoptosis. Acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism. Can reduce cell migration by regulating the expression of SPARC. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: PML body; autophagic vacuole; cytoplasmic vesicle; nucleus; cytosol

Molecular Function: antioxidant activity; protein binding

Biological Process: negative regulation of cell proliferation; transcription, DNA-dependent; response to heat; apoptosis; positive regulation of transcription, DNA-dependent; autophagic cell death; response to stress; cell cycle arrest; negative regulation of cell migration; positive regulation of autophagy; autophagic vacuole formation