Nuclear factor NF-kappa-B p105 subunit Recombinant Protein | NFKB1 recombinant protein

Recombinant Human Nuclear factor NF-kappa-B p105 subunit protein

Cat. Num. AAA953161

• Gene Names: NFKB1; p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nuclear factor NF-kappa-B p105 subunit; Recombinant Human Nuclear factor NF-kappa-B p105 subunit protein; DNA-binding factor KBF1EBP-1; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; NFKB1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 1-968aa; Full length
• Sequence: MAEDDPYLGRPEQMFHLDPSLTHTIFNPEVFQPQMALPTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGICTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHPDLAYLQAEGGGDRQLGDREKELIRQAALQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDINITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGGTGSTGPGYSFPHYGFPTYGGITFHPGTTKSNAGMKHGTMDTESKKDPEGCDKSDDKNTVNLFGKVIETTEQDQEPSEATVGNGEVTLTYATGTKEESAGVQDNLFLEKAMQLAKRHANALFDYAVTGDVKMLLAVQRHLTAVQDENGDSVLHLAIIHLHSQLVRDLLEVTSGLISDDIINMRNDLYQTPLHLAVITKQEDVVEDLLRAGADLSLLDRLGNSVLHLAAKEGHDKVLSILLKHKKAALLLDHPNGDGLNAIHLAMMSNSLPCLLLLVAAGADVNAQEQKSGRTALHLAVEHDNISLAGCLLLEGDAHVDSTTYDGTTPLHIAAGRGSTRLAALLKAAGADPLVENFEPLYDLDDSWENAGEDEGVVPGTTPLDMATSWQVFDILNGKPYEPEFTSDDLLAQGDMKQLAEDVKLQLYKLLEIPDPDKNWATLAQKLGLGILNNAFRLSPAPSKTLMDNYEVSGGTVRELVEALRQMGYTEAIEVIQAASSPVKTTSQAHSLPLSPASTRQQIDELRDSDSVCDSGVETSFRKLSFTESLTSGASLLTLNKMPHDYGQEGPLEGKI
• Sequence Length: 788

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for NFKB1 recombinant protein

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with mbers of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators. The NF-kappa-B p50-p50 homodimer is a transcriptional repressor, but can act as a transcriptional activator when associated with BCL3. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. The proteasome-mediated process ensures the production of both p50 and p105 and preserves their independent function, although processing of NFKB1/p105 also appears to occur post-translationally. p50 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. In a complex with MAP3K8, NFKB1/p105 represses MAP3K8-induced MAPK signaling; active MAP3K8 is released by proteasome-dependent degradation of NFKB1/p105.

Product Categories/Family for NFKB1 recombinant protein

Apoptosis

References

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Jr.Oncogene 9:1487-1492(1994) A glycine-rich region in NF-kappaB p105 functions as a processing signal for the generation of the p50 subunit.Lin L., Ghosh S.Mol. Cell. Biol. 16:2248-2254(1996) Inhibition of NF-kappaB DNA binding by nitric oxide.Matthews J.R., Botting C.H., Panico M., Morris H.R., Hay R.T.Nucleic Acids Res. 24:2236-2242(1996) A new member of the IkappaB protein family, IkappaB epsilon, inhibits RelA (p65) -mediated NF-kappaB transcription.Li Z., Nabel G.J.Mol. Cell. Biol. 17:6184-6190(1997) Cotranslational biogenesis of NF-kappaB p50 by the 26S proteasome.Lin L., De;Martino G.N., Greene W.C.Cell 92:819-828(1998) NF-kappaB p105 is a target of IkappaB kinases and controls signal induction of Bcl-3-p50 complexes.Heissmeyer V., Krappmann D., Wulczyn F.G., Scheidereit C.EMBO J. 18:4766-4778(1999) TPL-2 kinase regulates the proteolysis of the NF-kappaB-inhibitory protein NF-kappaB1 p105.Belich M.P., Salmeron A., Johnston L.H., Ley S.C.Nature 397:363-368(1999) Cotranslational dimerization of the Rel homology domain of NF-kappaB1 generates p50-p105 heterodimers and is required for effective p50 production.Lin L., De;Martino G.N., Greene W.C.EMBO J. 19:4712-4722(2000) RAC-3 is a NF-kappa B coactivator.Werbajh S., Nojek I., Lanz R., Costas M.A.FEBS Lett. 485:195-199(2000) Inhibition of NF-kappaB by S-nitrosylation.Marshall H.E., Stamler J.S.Biochemistry 40:1688-1693(2001) Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappa B.Ayroldi E., Migliorati G., Bruscoli S., Marchetti C., Zollo O., Cannarile L., D'Adamio F., Riccardi C.Blood 98:743-753(2001) 15-Deoxy-Delta 12,14-prostaglandin J2 inhibition of NF-kappaB-DNA binding through covalent modification of the p50 subunit.Cernuda-Morollon E., Pineda-Molina E., Canada F.J., Perez-Sala D.J. Biol. Chem. 276:35530-35536(2001) Direct phosphorylation of NF-kappa B1 p105 by the Ikappa B kinase complex on serine 927 is essential for signal-induced p105 proteolysis.Salmeron A., Janzen J., Soneji Y., Bump N., Kamens J., Allen H., Ley S.C.J. Biol. Chem. 276:22215-22222(2001) The tumor suppressor protein menin interacts with NF-kappaB proteins and inhibits NF-kappaB-mediated transactivation.Heppner C., Bilimoria K.Y., Agarwal S.K., Kester M., Whitty L.J., Guru S.C., Chandrasekharappa S.C., Collins F.S., Spiegel A.M., Marx S.J., Burns A.L.Oncogene 20:4917-4925(2001) Casper/c-FLIP is physically and functionally associated with NF-kappaB1 p105.Li Z., Zhang J., Chen D., Shu H.B.Biochem. Biophys. Res. Commun. 309:980-985(2003) Enhancement of nuclear factor-kappa B acetylation by coactivator p300 and HIV-1 Tat proteins.Furia B., Deng L., Wu K., Baylor S., Kehn K., Li H., Donnelly R., Coleman T., Kashanchi F.J. Biol. 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Biol. 24:9658-9667(2004) A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.Bouwmeester T., Bauch A., Ruffner H., Angrand P.-O., Bergamini G., Croughton K., Cruciat C., Eberhard D., Gagneur J., Ghidelli S., Hopf C., Huhse B., Mangano R., Michon A.-M., Schirle M., Schlegl J., Schwab M., Stein M.A., Bauer A., Casari G., Drewes G., Gavin A.-C., Jackson D.B., Joberty G., Neubauer G., Rick J., Kuster B., Superti-Furga G.Nat. Cell Biol. 6:97-105(2004) Posttranslational hydroxylation of ankyrin repeats in IkappaB proteins by the hypoxia-inducible factor (HIF) asparaginyl hydroxylase, factor inhibiting HIF (FIH) .Cockman M.E., Lancaster D.E., Stolze I.P., Hewitson K.S., McDonough M.A., Coleman M.L., Coles C.H., Yu X., Hay R.T., Ley S.C., Pugh C.W., Oldham N.J., Masson N., Schofield C.J., Ratcliffe P.J.Proc. Natl. Acad. Sci. 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Proteomics 96:253-262(2014) Structure of the NF-kappa B p50 homodimer bound to DNA.Mueller C.W., Rey F.A., Sodeoka M., Verdine G.L., Harrison S.C.Nature 373:311-317(1995) Structure of an IkappaBalpha/NF-kappaB complex.Jacobs M.D., Harrison S.C.Cell 95:749-758(1998) Solution structure of the death domain in human nuclear factor NF-kappa-B p105 subunit.RIKEN structural genomics initiative (RSGI) Submitted (DEC-2006) to the PDB data bank+Additional computationally mapped references.<p>Provides general information on the entry.

NCBI and Uniprot Product Information

• NCBI GI #: 259155302
• NCBI GeneID: 4790
• NCBI Accession #: NP_001158884.1
• NCBI GenBank Nucleotide #: NM_001165412.1
• UniProt Accession #: P19838; Q68D84; Q86V43; Q8N4X7; Q9NZC0; A8K5Y5; B3KVE8
• Molecular Weight: 132.4 kDa
• NCBI Official Full Name: nuclear factor NF-kappa-B p105 subunit isoform 2 proprotein
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
• NCBI Official Symbol: NFKB1
• NCBI Official Synonym Symbols: p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B
• NCBI Protein Information: nuclear factor NF-kappa-B p105 subunit
• UniProt Protein Name: Nuclear factor NF-kappa-B p105 subunit
• Protein Family: Nuclear factor
• UniProt Gene Name: NFKB1
• UniProt Entry Name: NFKB1_HUMAN

NCBI Description

This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. Alternative splicing results in multiple transcript variants encoding different isoforms, at least one of which is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

NFkB-p105: a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. There are five NFkB proteins in mammals (RelA/NFkB-p65, RelB, c-Rel, NF-_B1/NFkB-p105, and NF-_B2/NFkB-p100). They form a variety of homodimers and heterodimers, each of which activates its own characteristic set of genes. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: cytoplasm; cytosol; mitochondrion; neuron projection; nucleoplasm; nucleus

Molecular Function: actinin binding; chromatin binding; heat shock protein binding; identical protein binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription factor activity; transcription factor binding

Biological Process: activation of NF-kappaB transcription factor; apoptosis; I-kappaB kinase/NF-kappaB cascade; inflammatory response; innate immune response; membrane protein intracellular domain proteolysis; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of cellular protein metabolic process; negative regulation of cholesterol transport; negative regulation of inflammatory response; negative regulation of interleukin-12 biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; nerve growth factor receptor signaling pathway; positive regulation of interferon type I production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to copper ion; response to oxidative stress; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; T cell receptor signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; transcription from RNA polymerase II promoter

Disease: Immunodeficiency, Common Variable, 12