Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.)
Mouse AMIGO-1 Monoclonal Antibody | anti-AMIGO1 antibody
AMIGO-1 Antibody: ATTO 488
Gene Names
AMIGO1; ALI2; AMIGO; AMIGO-1
Reactivity
Mouse, Human, Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry
Synonyms
AMIGO-1; Monoclonal Antibody; AMIGO-1 Antibody: ATTO 488; AMIGO 1; AMIGO1; Adhesion molecule with Ig like domain 1; Amphoterin-induced protein 1; Alivin-2; Alivin 2; Ali2; AMIGO; KIAA1163; Amphoterin induced gene and ORF (Amigo); Amphoterin induced protein 1; MGC25558; OTTHUMP00000013379; RP23 89M15.6; anti-AMIGO1 antibody
Host
Mouse
Reactivity
Mouse, Human, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
S86-36
Form/Format
Protein G Purified
Concentration
1mg/mL (varies by lot)
Sequence Length
493
Applicable Applications for anti-AMIGO1 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
Application Notes
1:1000 (WB)
Conjugate
ATTO 488
Storage Buffer
PBS pH7.4, 50% glycerol, 0.09% sodium azide
Preparation and Storage
-20 degree C
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.)
Western Blot (WB)
(Western Blot analysis of Rat Brain Membrane showing detection of 60-80 kDa AMIGO-1 protein using Mouse Anti-AMIGO-1 Monoclonal Antibody, Clone S86-36. Lane 1: Molecular Weight Ladder. Lane 2: Rat Brain Membrane. Load: 15 ug. Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-AMIGO-1 Monoclonal Antibody at 1:200 for 16 hours at 4 degree C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 60-80 kDa.)
Related Product Information for anti-AMIGO1 antibody
Background Info: Detects 60-80kDa depending on maturity/glycosylation. Does not bind AMIGO-2 or 3.
Scientific Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
Scientific Background: The amphoterin-induced gene and ORF (AMIGO) family of proteins consists of AMIGO1, AMIGO2 and AMIGO3. All three members are single pass type I membrane proteins that contain several leucine-rich repeats, one IgG domain and a transmembrane domain. The AMIGO proteins are specifically expressed on fiber tracts of neuronal tissues and participate in their formation. They can form complexes with each other, but can also self-bind. AMIGO1, also designated Alivin2, promotes growth and fasciculation of neurites and plays a role in myelination and fasciculation of developing neural axons. In cerebellar neurons, AMIGO2 (Alivin1) is crucial for depolarization-dependent survival. Similar to AMIGO1 and AMIGO2, AMIGO3 (Alivin3) plays a role in hemophilic and/or heterophilic cell-cell interaction and signal transduction.
Product Categories/Family for anti-AMIGO1 antibody
References
1. Kuja-Panula J., Kiiltomäki M., Yamashiro T., Rouhiainen A. and Rauvala H. (2003) J. Cell Biol. 160: 963-973. 2. Clark H.F., et al. (2003) Genome Res. 13: 2265-2270. 3. On, T., Sekino-Suzuki N., Kikkawa Y., Yonekawa H. and Kawashima S. (2003) J. Neurosci. 23: 5887-5896. 4. Chen Y., Aulia S., Li L. and Tang B.L. (2006) Brain Res. Brain Res. Rev. 51: 265-274.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,239 Da
NCBI Official Full Name
amphoterin-induced protein 1
NCBI Official Synonym Full Names
adhesion molecule with Ig-like domain 1
NCBI Official Symbol
AMIGO1
NCBI Official Synonym Symbols
ALI2; AMIGO; AMIGO-1
NCBI Protein Information
amphoterin-induced protein 1; alivin-2; amphoterin-induced gene and ORF
UniProt Protein Name
Amphoterin-induced protein 1
Protein Family
UniProt Gene Name
AMIGO1
UniProt Synonym Gene Names
ALI2; AMIGO; KIAA1163
UniProt Entry Name
AMGO1_HUMAN
Uniprot Description
AMIGO1: Promotes growth and fasciculation of neurites from cultured hippocampal neurons. May be involved in fasciculation as well as myelination of developing neural axons. May have a role in regeneration as well as neural plasticity in the adult nervous system. May mediate homophilic as well as heterophilic cell-cell interaction and contribute to signal transduction through its intracellular domain. Belongs to the immunoglobulin superfamily. AMIGO family.
Protein type: Cell development/differentiation; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p13.3
Cellular Component: axon; plasma membrane; integral to membrane
Biological Process: heterophilic cell adhesion; myelination; axonogenesis; axonal fasciculation; positive regulation of axonogenesis; brain development; homophilic cell adhesion
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Product Notes
The AMIGO1 amigo1 (Catalog #AAA803538) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AMIGO-1 Antibody: ATTO 488 reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AMIGO-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC). 1:1000 (WB). Researchers should empirically determine the suitability of the AMIGO1 amigo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMIGO-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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