Rabbit anti-Human, Mouse TOP3B Polyclonal Antibody | anti-TOP3B antibody

TOP3B, CT (TOP3B, TOP3B1, DNA topoisomerase 3-beta-1, DNA topoisomerase III beta-1) (Biotin)

Cat. Num. AAA6357375

• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TOP3B; Polyclonal Antibody; CT (TOP3B; TOP3B1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta-1) (Biotin); DNA topoisomerase III beta-1; anti-TOP3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TOP3B antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TOP3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 825-854 amino acids from the C-terminal region of human TOP3B.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TOP3B Antibody (C-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the TOP3B antibody detected the TOP3B protein (arrow).)

Related Product Information for anti-TOP3B antibody

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described.

Product Categories/Family for anti-TOP3B antibody

Antibodies; Topoisomerases

References

Oliveira-Costa, J.P., et al. Hum. Pathol. 41(11):1624-1630(2010). Lesch, K.P., et al. J Neural Transm 115(11):1573-1585(2008). Cho, Y.H., et al. Biochim. Biophys. Acta 1679(3):272-278(2004). Lehner, B., et al. Genome Res. 14(7):1315-1323(2004). Collins, J.E., et al. Genome Biol. 5 (10), R84 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 4507635
• NCBI GeneID: 8940
• NCBI Accession #: NP_003926.1
• NCBI GenBank Nucleotide #: NM_003935.3
• UniProt Accession #: O95985; Q9BUP5; A0M8Q3
• Molecular Weight: 96,662 Da
• NCBI Official Full Name: DNA topoisomerase 3-beta-1
• NCBI Official Synonym Full Names: topoisomerase (DNA) III beta
• NCBI Official Symbol: TOP3B
• NCBI Protein Information: DNA topoisomerase 3-beta-1; topoisomerase III beta; DNA topoisomerase III beta-1
• UniProt Protein Name: DNA topoisomerase 3-beta-1
• Protein Family: DNA topoisomerase
• UniProt Gene Name: TOP3B
• UniProt Synonym Gene Names: TOP3B1
• UniProt Entry Name: TOP3B_HUMAN

NCBI Description

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]

Uniprot Description

TOP3B: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity. Belongs to the type IA topoisomerase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 5.99.1.2; DNA-binding; Topoisomerase

Chromosomal Location of Human Ortholog: 22q11.22

Cellular Component: nucleoplasm; condensed chromosome; nucleus

Molecular Function: DNA binding; DNA topoisomerase activity; DNA topoisomerase type I activity

Biological Process: DNA topological change; chromosome segregation