Goat TNNT3 Polyclonal Antibody | anti-TNNT3 antibody

Goat Polyclonal to Human TNNT3

Cat. Num. AAA248667

• Gene Names: TNNT3; TNTF
• Reactivity: Chimpanzee, Guinea pig, Gorilla, Human, Monkey, Mouse, Orangutan, Rat
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: TNNT3; Polyclonal Antibody; Goat Polyclonal to Human TNNT3; Anti-TNNT3 Antibody (C-Terminus) IHC-plus; AMCD2B; Beta-TnTF; DA2B; FTnT; FSSV; TNTF; Troponin-T3; skeletal; fast; Beta TnTF; Troponin T3; Human TNNT3; anti-TNNT3 antibody

• Host: Goat
• Reactivity: Chimpanzee, Guinea pig, Gorilla, Human, Monkey, Mouse, Orangutan, Rat
• Clonality: Polyclonal
• Specificity: Human TNNT3. This antibody is expected to recognize isoform 1, 2 and 4 (NP_006748.1; NP_001036246.1; NP_001036247.1).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 258

• Applicable Applications for anti-TNNT3 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (5 ug/ml), WB (0.01 - 0.03 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 35kD band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 30.6kD according to NP_006748.1). The observed molecular weight corresponds to earlie...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-TTLRSRIDQAQKHSK from the C-terminus of human TNNT3 (NP_006748.1; NP_001036246.1; NP_001036247.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Baboon, Monkey, Marmoset, Mouse, Rat, Guinea pig (100%).
• Immunogen Type: Synthetic peptide
• Immunogen: TNNT3 antibody was raised against synthetic peptide C-TTLRSRIDQAQKHSK from the C-terminus of human TNNT3 (NP_006748.1; NP_001036246.1; NP_001036247.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Baboon, Monkey, Marmoset, Mouse, Rat, Guinea pig (100%); Galago, Shrew, Goat, Bat, Dog, Cat, Bovine, Rabbit, Lizard (93%); Ferret, Horse, Pig, Turkey, Zebra finch, Chicken (87%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-TNNT3 antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(TNNT3 antibody (0.01 ug/ml) staining of Human (A), Mouse (B) and Rat (C) Skeletal Muscle lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 5803203
• NCBI GeneID: 7140
• NCBI Accession #: NP_006748.1
• NCBI GenBank Nucleotide #: NM_006757.3
• UniProt Accession #: P45378; Q12975; Q12976; Q12977; Q12978; Q17RG9; A8MQ76; A8MSW1; B3KPX3; B7WP64; B7ZL26; B7ZVV9
• Molecular Weight: 29,735 Da
• NCBI Official Full Name: troponin T, fast skeletal muscle isoform 1
• NCBI Official Synonym Full Names: troponin T type 3 (skeletal, fast)
• NCBI Official Symbol: TNNT3
• NCBI Official Synonym Symbols: TNTF
• NCBI Protein Information: troponin T, fast skeletal muscle; fTnT; beta TnTF; beta-TnTF; troponin-T3, skeletal, fast; fast skeletal muscle troponin T
• UniProt Protein Name: Troponin T, fast skeletal muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT3
• UniProt Synonym Gene Names: TnTf; fTnT
• UniProt Entry Name: TNNT3_HUMAN

NCBI Description

The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009]

Uniprot Description

TNNT3: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Defects in TNNT3 are a cause of distal arthrogryposis type 2B (DA2B); also known as arthrogryposis multiplex congenita, distal, type 2B (AMCD2B). DA2B is a form of inherited multiple congenital contractures. Affected individuals have vertical talus, ulnar deviation in the hands, severe camptodactyly, and a distinctive face characterized by a triangular shape, prominent nasolabial folds, small mouth and a prominent chin. Belongs to the troponin T family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: troponin complex; cytosol

Molecular Function: troponin C binding; troponin I binding; actin binding; tropomyosin binding; calcium-dependent protein binding; calcium-dependent ATPase activity

Biological Process: skeletal muscle contraction; regulation of ATPase activity; metabolic process; regulation of striated muscle contraction; muscle filament sliding

Disease: Arthrogryposis, Distal, Type 2b