Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TNRC6A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit TNRC6A Polyclonal Antibody | anti-TNRC6A antibody
TNRC6A Polyclonal Antibody
IF: 1:50 - 1:100
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TNRC6A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TNRC6A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OScells using TNRC6A antibody (MBS9128165) atdilution of 1:100. Blue: DAPI for nuclearstaining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OScells using TNRC6A antibody (MBS9128165) atdilution of 1:100. Blue: DAPI for nuclearstaining.)
NCBI and Uniprot Product Information
Calculated MW: 17kda/36kDa/182kDa/205kDa/219kDa
NCBI Description
This gene encodes a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. [provided by RefSeq, Jul 2008]
Uniprot Description
TNRC6A: Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA- dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins. Belongs to the GW182 family. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Translation
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: nucleoplasm; cytosol
Molecular Function: protein binding; nucleotide binding
Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; miRNA-mediated gene silencing, negative regulation of translation; innate immune response; gene expression; miRNA-mediated gene silencing; cellular response to starvation; posttranscriptional gene silencing
Research Articles on TNRC6A
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Product Notes
The TNRC6A tnrc6a (Catalog #AAA9128165) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's TNRC6A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB 1:500 - 1:1000 IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the TNRC6A tnrc6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNRC6A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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