Mouse anti-Human MAP3K12 Monoclonal Antibody | anti-MAP3K12 antibody

MAP3K12 (MAP3K12, ZPK, Mitogen-activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase) (APC)

Cat. Num. AAA6257507

• Gene Names: MAP3K12; DLK; MUK; ZPK; ZPKP1; MEKK12
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: MAP3K12; Monoclonal Antibody; MAP3K12 (MAP3K12; ZPK; Mitogen-activated protein kinase kinase kinase 12; Dual leucine zipper bearing kinase; Leucine-zipper protein kinase; MAPK-upstream kinase; Mixed lineage kinase) (APC); Mixed lineage kinase; anti-MAP3K12 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 473CT23.12.5
• Specificity: Recognizes human MAP3K12.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-MAP3K12 antibody: FLISA, Western Blot (WB)
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 828-859 of human MAP3K12.
• Conjugate: APC
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Western Blot (WB)

(Western Blot analysis in T47D cell line lysates (35ug/lane) usingMBS645852. This demonstrates MBS645852 detected the MAP3K12 protein (arrow).)

Product Categories/Family for anti-MAP3K12 antibody

Antibodies; Protein Kinases

References

1. Clague, J., et al. Mol. Carcinog. 49(2):183-189(2010) 2. Robitaille, H., et al. J. Invest. Dermatol. 130(1):74-85(2010) 3. Landa, I., et al. PLoS Genet. 5 (9), E1000637 (2009) 4. Daviau, A., et al. Cell. Signal. 21(4):577-587(2009) 5. Mukhopadhyay, R., et al. Mol. Cell 32(3):371-382(2008)

NCBI and Uniprot Product Information

• NCBI GI #: 301898343
• NCBI GeneID: 7786
• NCBI Accession #: NP_001180440.1
• NCBI GenBank Nucleotide #: NM_001193511.1
• UniProt Accession #: Q12852; Q86VQ5; Q8WY25; B3KSS9; G3V1Y2
• Molecular Weight: 96,322 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 12 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 12
• NCBI Official Symbol: MAP3K12
• NCBI Official Synonym Symbols: DLK; MUK; ZPK; ZPKP1; MEKK12
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 12; protein kinase MUK; MAPK-upstream kinase; mixed lineage kinase; leucine zipper protein kinase; dual leucine zipper kinase DLK; dual leucine zipper bearing kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 12
• Protein Family: MAP3K12-binding inhibitory protein
• UniProt Gene Name: MAP3K12
• UniProt Synonym Gene Names: ZPK; DLK; ZPK; MUK
• UniProt Entry Name: M3K12_HUMAN

Uniprot Description

Function: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Subunit structure: Interacts with MBIP. Ref.7

Subcellular location: Cytoplasm

By similarity. Membrane; Peripheral membrane protein

By similarity.

Tissue specificity: Highly expressed in brain and kidney.

Domain: Interacts with MBIP through the leucine-zipper motif.

Post-translational modification: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated

By similarity.

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.