Rabbit anti-Human TNRC4 Polyclonal Antibody | anti-TNRC4 antibody

TNRC4, NT (CELF3, BRUNOL1, CAGH4, ERDA4, TNRC4, CUGBP Elav-like family member 3, Bruno-like protein 1, CAG repeat protein 4, CUG-BP-and ETR-3-like factor 3, ELAV-type RNA-binding protein 1, Expanded repeat domain protein CAG/CTG 4, RNA-binding protein BRU

Cat. Num. AAA6357287

• Gene Names: CELF3; CAGH4; ERDA4; ETR-1; TNRC4; BRUNOL1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TNRC4; Polyclonal Antibody; NT (CELF3; BRUNOL1; CAGH4; ERDA4; CUGBP Elav-like family member 3; Bruno-like protein 1; CAG repeat protein 4; CUG-BP-and ETR-3-like factor 3; ELAV-type RNA-binding protein 1; Expanded repeat domain protein CAG/CTG 4; RNA-binding protein BRU; CELF3; RNA-binding protein BRUNOL-1; Trinucleotide repeat-containing gene 4 protein; anti-TNRC4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-TNRC4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: TNRC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human TNRC4.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(TNRC4 Antibody (N-term) western blot analysis in Jurkat,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the TNRC4 antibody detected the TNRC4 protein (arrow).)

Flow Cytometry (FC/FACS)

(TNRC4 Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TNRC4 antibody

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Product Categories/Family for anti-TNRC4 antibody

Antibodies; Binding Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). Chapple, J.P., et al. Hum. Mol. Genet. 16(22):2760-2769(2007). Ladd, A.N., et al. Mol. Cell. Biol. 21(4):1285-1296(2001). Good, P.J., et al. J. Biol. Chem. 275(37):28583-28592(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 71164894
• NCBI GeneID: 11189
• NCBI Accession #: NP_009116.3
• NCBI GenBank Nucleotide #: NM_007185.6
• UniProt Accession #: Q5SZQ8; O15414; Q499Y6; Q5SZQ7; Q6NVK0; Q8IZ98; Q9BZC2; Q9HB30; B7ZKK6
• Molecular Weight: 45,686 Da
• NCBI Official Full Name: CUGBP Elav-like family member 3 isoform 1
• NCBI Official Synonym Full Names: CUGBP, Elav-like family member 3
• NCBI Official Symbol: CELF3
• NCBI Official Synonym Symbols: CAGH4; ERDA4; ETR-1; TNRC4; BRUNOL1
• NCBI Protein Information: CUGBP Elav-like family member 3; CAG repeat domain; CAG repeat protein 4; bruno-like protein 1; RNA-binding protein BRUNOL-1; CUG-BP and ETR-3 like factor 3; CUG-BP- and ETR-3-like factor 3; ELAV-type RNA-binding protein 1; expanded repeat domain, CAG/CTG
• UniProt Protein Name: CUGBP Elav-like family member 3
• UniProt Gene Name: CELF3
• UniProt Synonym Gene Names: BRUNOL1; CAGH4; ERDA4; TNRC4; CELF-3; ETR-1
• UniProt Entry Name: CELF3_HUMAN

NCBI Description

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Feb 2010]

Uniprot Description

TNRC4: RNA-binding protein involved in the regulation of pre- mRNA alternative splicing. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Activates the splicing of MAPT/Tau exon 10. Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Belongs to the CELF/BRUNOL family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: RNA splicing

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoplasm; nucleus

Molecular Function: mRNA binding; RNA binding; nucleotide binding

Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; sperm motility; RNA splicing; spermatogenesis; regulation of alternative nuclear mRNA splicing, via spliceosome