Immunohistochemistry (IHC)
(Anti-TNR antibody, MBS176385, IHC(P)IHC(P): Rat Brain Tissue )
Rabbit TNR Polyclonal Antibody | anti-TNR antibody
Anti-TNR antibody
Concentration: 0.1-0.5 ug/mL
Tested Species: Hu, Ms, Rat
Immunohistochemistry (Paraffin-embedded Section)
Concentration: 0.5-1 ug/mL
Tested Species: Rat
Predicted Species: Hu, Ms
Antigen Retrieval : By Heat
WB: The detection limit for TNR is approximately 3 ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. .
Other applications have not been tested.
Optimal dilutions should be determined by end users.
Immunohistochemistry (IHC)
(Anti-TNR antibody, MBS176385, IHC(P)IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-TNR antibody, MBS176385, IHC(P)IHC(P): Rat Brain Tissue )
Western Blot (WB)
(Anti-TNR antibody, MBS176385, Western blottingAll lanes: Anti TNR (MBS176385) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 150KDObserved bind size: 150KD)
Western Blot (WB)
(Anti-TNR antibody, MBS176385, Western blottingAll lanes: Anti TNR (MBS176385) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: U87 Whole Cell Lysate at 40ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: MCF-7 Whole Cell Lysate at 40ugPredicted bind size: 150KDObserved bind size: 150KD)
Background: Tenascin-R is a protein that in humans is encoded by the TNR gene. Tenascin-R(TNR) is an extracellular matrix protein expressed primarily in the central nervous system. It is a member of the tenascin(TN) gene family, which includes at least 3 genes in mammals: TNC(or hexabrachion), TNX(TNXB), and TNR. The genes are expressed in distinct tissues at different times during embryonic development and are present in adult tissues. TNR has been detected predominantly in the central nervous system and is localized around motor neurons and on motor axons in the spinal cord, cerebellum, hippocampus, and olfactory bulb. It is suggested that tenascin-R has a role in initiating the detachment of neuroblasts from tangential chains and in initiating radial migration of the cells.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The encoded protein is restricted to the central nervous system. The protein may play a role in neurite outgrowth, neural cell adhesion and modulation of sodium channel function. It is a constituent of perineuronal nets. [provided by RefSeq, Aug 2013]
Uniprot Description
TNR: Neural extracellular matrix (ECM) protein involved in interactions with different cells and matrix components. These interactions can influence cellular behavior by either evoking a stable adhesion and differentiation, or repulsion and inhibition of neurite growth. Binding to cell surface gangliosides inhibits RGD-dependent integrin-mediated cell adhesion and results in an inhibition of PTK2/FAK1 (FAK) phosphorylation and cell detachment. Binding to membrane surface sulfatides results in a oligodendrocyte adhesion and differentiation. Interaction with CNTN1 induces a repulsion of neurons and an inhibition of neurite outgrowth. Interacts with SCN2B may play a crucial role in clustering and regulation of activity of sodium channels at nodes of Ranvier. TNR-linked chondroitin sulfate glycosaminoglycans are involved in the interaction with FN1 and mediate inhibition of cell adhesion and neurite outgrowth. The highly regulated addition of sulfated carbohydrate structure may modulate the adhesive properties of TNR over the course of development and during synapse maintenance. Belongs to the tenascin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q24
Cellular Component: nucleoplasm; proteinaceous extracellular matrix; cell surface; cytoplasm; extracellular region; nucleus; lipid raft
Biological Process: negative regulation of axon extension involved in regeneration; axon guidance; extracellular matrix organization and biogenesis; synapse organization and biogenesis; neuron adhesion; negative regulation of cell-cell adhesion; cell adhesion; neuromuscular process controlling balance; positive regulation of synaptic transmission, glutamatergic; negative regulation of synaptic transmission; associative learning; telencephalon cell migration; positive regulation of transmission of nerve impulse
Research Articles on TNR
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Product Notes
The TNR tnr (Catalog #AAA176385) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TNR antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNR can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Western Blot Concentration: 0.1-0.5 ug/mL Tested Species: Hu, Ms, Rat Immunohistochemistry (Paraffin-embedded Section) Concentration: 0.5-1 ug/mL Tested Species: Rat Predicted Species: Hu, Ms Antigen Retrieval : By Heat WB: The detection limit for TNR is approximately 3 ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Researchers should empirically determine the suitability of the TNR tnr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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