Rabbit TNNT2 Polyclonal Antibody | anti-TNNT2 antibody

TNNT2 Polyclonal Antibody

Cat. Num. AAA3013590

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Peptide affinity purified
• Synonyms: TNNT2; Polyclonal Antibody; TNNT2 Polyclonal Antibody; TnTc; cTnT; Cardiac muscle troponin T; Cardiomyopathy dilated 1D (autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T; cardiac muscle; Troponin T2 cardiac; anti-TNNT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNNT2 polyclonal antibody detects endogenous levels of TNNT2 protein.
• Purity/Purification: Peptide affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 288

• Applicable Applications for anti-TNNT2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:5000; IHC: 1:200
• Immunogen: Synthetic peptide (KLH-coupled) within human TNNT2 1-100 aa.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of TNNT2 on different lysates using anti-TNNT2 antibody at 1/5,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-TNNT2 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-TNNT2 antibody

Troponin T type 2 (cardiac), also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations of this gene may be associated with mild or absent hypertrophy and predominant restrictive disease, with a high risk of sudden cardiac death.

NCBI and Uniprot Product Information

• NCBI GI #: 15290516
• NCBI GeneID: 7139
• UniProt Accession #: P45379; P50752; P50753
• Molecular Weight: 36kDa
• NCBI Official Full Name: cardiac troponin T
• NCBI Official Synonym Full Names: troponin T2, cardiac type
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: positive regulation of ATPase activity; regulation of muscle contraction; atrial cardiac muscle morphogenesis; metabolic process; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2