Rabbit TNNT2 Polyclonal Antibody | anti-TNNT2 antibody

TNNT2 Polyclonal Antibody

Cat. Num. AAA3006403

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: TNNT2; Polyclonal Antibody; TNNT2 Polyclonal Antibody; Cardiac muscle troponin T; Cardiomyopathy dilated 1D (autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; TnTC; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T; cardiac muscle; Troponin T2; Troponin T2 cardiac; anti-TNNT2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: TNNT2 polyclonal antibody detects endogenous levels of TNNT2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 288

• Applicable Applications for anti-TNNT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant full length Human TNNT2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of TNNT2 polyclonal antibody.)

Related Product Information for anti-TNNT2 antibody

Actin is a highly conserved protein that is expressed in all eukaryotic cells. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. Myosin is a hexamer of two heavy chains (MHC) and four light chains (MLC) that interacts with Actin to generate the force for diverse cellular movements, including cytokinesis, phagocytosis and muscle contraction. Troponin facilitates the interaction between Actin and Myosin by binding to calcium. Troponin is made up of at least two subunits, which are divergent in cardiac muscle, fast skeletal muscle and slow skeletal muscle. Structures of skeletal muscle troponin are composed of Troponin C (the sensor), Troponin I (the regulator) and Troponin T (the link to the muscle thin filament). Troponin C is dumbbell-shaped and has a hydrophobic pocket that increases the contractile force of muscle fibers. Troponin C has two isoforms: fast and slow. Fast Troponin C has two calcium binding sites, while slow/cardiac Troponin C has a single calcium binding site.

NCBI and Uniprot Product Information

• NCBI GI #: 15290516
• NCBI GeneID: 7139
• UniProt Accession #: P45379
• Molecular Weight: ~ 45kDa
• NCBI Official Full Name: cardiac troponin T
• NCBI Official Synonym Full Names: troponin T2, cardiac type
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT
• UniProt Entry Name: TNNT2_HUMAN

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament

Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding

Biological Process: positive regulation of ATPase activity; regulation of muscle contraction; atrial cardiac muscle morphogenesis; metabolic process; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding

Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2