CST6 recombinant protein

CST6 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123025

• Gene Names: Cst6; ichq; N28197; 1110017E11Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: CST6; CST6 Protein; Mouse; Recombinant (His Tag); Mouse Cystatin E/CST6 Protein (His Tag); 1110017E11Rik Protein; ichq Protein; N28197 Protein; cystatin E/M; CST6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala152

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CST6 (NP_082899.1)(Met 1-Ala 152) was expressed, with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CST6 recombinant protein

Background: Cystatin E/M, also referred to as CST6, is a member of type 2 cysteine proteinase inhibitors of the cystatin superfamily, and inhibits papain and cathepsin B. Cystatin E is a low molecular mass secreted protein existing in both a glycosylated (17kDa) and an unglycosylated (14kDa) form, with two characteristic intrachain disulfide bridges. Expression of cystatin M/E is found to be restricted to the epidermis, more specifically in the stratum granulosum, sweat glands, sebaceous glands, and the hair follicles. In addition to its function as a cysteine protease inhibitor, cystatin M/E also serves as a target for cross-linking by transglutaminases. Accordingly, cystatin M/E was suggested to be involved in barrier formation and maintenance. Furthermore, studies have revealed that cystatin M/E is frequently epigenetically inactivated during breast carcinogenesis, and thus be regarded as a candidate of tumour suppressor gene.

References

Ritonja A., et al.,(1985), Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver. Biochem. Biophys. Res. Commun. 131:1187-1192.Pennacchio L.A., et al., (1996), Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1).Science 271:1731-1734.Ghaemmaghami S., et al.,(2003), Global analysis of protein expression in yeast.Nature 425:737-741.

NCBI and Uniprot Product Information

• NCBI GI #: 21312060
• NCBI GeneID: 73720
• NCBI Accession #: NP_082899.1
• NCBI GenBank Nucleotide #: NM_028623.5
• UniProt Accession #: Q9D1B1
• Molecular Weight: 16,796 Da
• NCBI Official Full Name: cystatin-M
• NCBI Official Synonym Full Names: cystatin E/M
• NCBI Official Symbol: Cst6
• NCBI Official Synonym Symbols: ichq; N28197; 1110017E11Rik
• NCBI Protein Information: cystatin-M; cystatin M; harlequin ichthyosis; harlequin ichthysosis
• UniProt Protein Name: Cystatin E/M
• Protein Family: Cystatin
• UniProt Gene Name: Cst6
• UniProt Entry Name: Q9D1B1_MOUSE

Uniprot Description

CST6: Shows moderate inhibition of cathepsin B but is not active against cathepsin C. Belongs to the cystatin family.

Protein type: Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide

Cellular Component: cornified envelope

Biological Process: epidermis development