Rabbit anti-Human, Mouse TNFRSF25 / DR3 Polyclonal Antibody | anti-TNFRSF25 antibody

Rabbit Polyclonal to Human TNFRSF25 / DR3

Cat. Num. AAA242166

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
• Purity: Ion exchange Chromatography
• Synonyms: TNFRSF25 / DR3; Polyclonal Antibody; Rabbit Polyclonal to Human TNFRSF25 / DR3; Anti-TNFRSF25 / DR3 Antibody (Extracellular Domain) IHC-plus; TNFRSF25; APO-3; APO3; DDR3; Death receptor 3; DR3; Death receptor beta; LARD; Protein WSL; TR3; WSL; WSL-1; WSL-LR; Apoptosis inducing receptor; TNFRSF12; WSL1; Protein WSL-1; TRAMP; Human TNFRSF25; anti-TNFRSF25 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: Peptide corresponding to amino acids in extracellular domain (ED) of human DR3 precursor.
• Purity/Purification: Ion exchange Chromatography
• Form/Format: PBS, 0.02% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 417

• Applicable Applications for anti-TNFRSF25 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, ICC (10 ug/ml), IHC-P (3 ug/ml), WB (1:500 - 1:1000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human TNFRSF25 / DR3.
• Immunogen Type: Synthetic peptide
• Immunogen: TNFRSF25 / DR3 antibody was raised against synthetic peptide from human TNFRSF25 / DR3.
• Antigen Modification: Extracellular Domain
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-DR3 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of DR3 in Jurkat cells with DR3 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 µg/ml.)

Western Blot (WB)

(Western blot of DR3 in Jurkat total cell lysate with DR3 antibody at 1 ug/ml.)

Related Product Information for anti-TNFRSF25 antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L.

Product Categories/Family for anti-TNFRSF25 antibody

Family: TNF Receptor

NCBI and Uniprot Product Information

• NCBI GI #: 4507569
• NCBI GeneID: 8718
• NCBI Accession #: NP_003781.1
• NCBI GenBank Nucleotide #: NM_003790.2
• UniProt Accession #: Q93038; O00275; O00276; O00277; O00278; O00279; O00280; O14865; O14866; B1ALX2; B1ALX3; B7ZLL7
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 2
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25; protein WSL-1; death receptor beta; apoptosis inducing receptor; apoptosis-inducing receptor AIR; apoptosis-mediating receptor DR3; apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; lymphocyte-associated receptor of death; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Apoptosis; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction