Rabbit anti-Human, Mouse DR3 Polyclonal Antibody | anti-DR3 antibody

DR3 Antibody

Cat. Num. AAA9402075

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: DEAE purified
• Synonyms: DR3; Polyclonal Antibody; DR3 Antibody; Wsl-1 Apo-3; anti-DR3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Purity/Purification: DEAE purified
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 417

• Applicable Applications for anti-DR3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids in extracellular domain of human DR3 precusor.
• Target Name: DR3
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of DR3 in Jurkat total cell lysate with DR3 antibody at 1:1000 dilution.)

Immunocytochemistry (ICC)

(Immunocytochemistry of DR3 in Jurkat cells with DR3 antibody at 10 ug/mL.)

Related Product Information for anti-DR3 antibody

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including TNF and Fas ligand of the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel cell death receptor was recently identified by several groups independently and designated DR3, Wsl-1, Apo-3, TRAMP and LARD1-5. The ligand for this novel death receptor has been defined as TWEAK, also termed Apo3L. DR3 is highly expressed in the tissues enriched in lymphocytes including PBL, thymus and spleen. Like TNFR1, DR3 induces apoptosis and NF-kB activation.

Product Categories/Family for anti-DR3 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 37181738
• NCBI GeneID: 8718
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: DR3
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: cytosol; extracellular region; integral to plasma membrane; plasma membrane

Molecular Function: receptor activity; tumor necrosis factor receptor activity

Biological Process: apoptosis; cell surface receptor linked signal transduction; immune response; inflammatory response; multicellular organismal development; positive regulation of MAPKKK cascade; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway