Rabbit DR3 Polyclonal Antibody | anti-DR3 antibody

RABBIT ANTI HUMAN DR3 (N-TERMINAL)

Cat. Num. AAA221574

• Gene Names: TNFRSF25; DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• Applications: Western Blot
• Synonyms: DR3; Polyclonal Antibody; RABBIT ANTI HUMAN DR3 (N-TERMINAL); anti-DR3 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises a 59kD apoptosis related protein designated DR3 (death receptor-3).DR3 is a cell surface receptor with a death domain, with functional similarities to TNFR1 and Fas (CD95).The ligand for DR3 has very recently been identified and has been termed Apo-3L.DR3 itself has also been called Wsl-1, Apo-3, TRAMP and LARD, and functionally induces apoptosis and NF-KB activation.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-DR3 antibody: Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Peptide sequence QGGTRSPRCDCAGDFH from amino terminus of human DR3; Buffer Solution
• Antiserum Preparation: Antisera to DR3 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysate from Jurkat cells probed with Rabbit anti Human DR3 (MBS221574))

Related Product Information for anti-DR3 antibody

MBS221574 recognises a 59kD apoptosis related protein designated DR3 (death receptor-3).DR3 is a cell surface receptor with a death domain, with functional similarities to TNFR1 and Fas (CD95). The ligand for DR3 has very recently been identified and has been termed Apo-3L. DR3 itself has also been called Wsl-1, Apo-3, TRAMP and LARD, and functionally induces apoptosis and NF-KB activation.

NCBI and Uniprot Product Information

• NCBI GI #: 89142745
• NCBI GeneID: 8718
• NCBI Accession #: NP_001034753.1
• NCBI GenBank Nucleotide #: NM_001039664.1
• UniProt Accession #: Q93038; O00275; O00276; O00277; O00278; O00279; O00280; O14865; O14866; B1ALX2; B1ALX3; B7ZLL7
• Molecular Weight: 29,111 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 25 isoform 12
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 25
• NCBI Official Symbol: TNFRSF25
• NCBI Official Synonym Symbols: DR3; TR3; DDR3; LARD; APO-3; TRAMP; WSL-1; WSL-LR; TNFRSF12
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 25; apoptosis inducing receptor; apoptosis-inducing receptor AIR; apoptosis-mediating receptor DR3; apoptosis-mediating receptor TRAMP; death domain receptor 3 soluble form; death receptor beta; lymphocyte-associated receptor of death; protein WSL-1; tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 25
• UniProt Gene Name: TNFRSF25
• UniProt Synonym Gene Names: APO3; DDR3; DR3; TNFRSF12; WSL; WSL1; LARD
• UniProt Entry Name: TNR25_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is expressed preferentially in the tissues enriched in lymphocytes, and it may play a role in regulating lymphocyte homeostasis. This receptor has been shown to stimulate NF-kappa B activity and regulate cell apoptosis. The signal transduction of this receptor is mediated by various death domain containing adaptor proteins. Knockout studies in mice suggested the role of this gene in the removal of self-reactive T cells in the thymus. Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported, most of which are potentially secreted molecules. The alternative splicing of this gene in B and T cells encounters a programmed change upon T-cell activation, which predominantly produces full-length, membrane bound isoforms, and is thought to be involved in controlling lymphocyte proliferation induced by T-cell activation. [provided by RefSeq, Jul 2008]

Uniprot Description

TNFRSF25: Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis. Homodimer. Interacts strongly via the death domains with TNFRSF1 and TRADD to activate at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4. Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate. 12 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: integral to plasma membrane; extracellular region; plasma membrane; cytosol

Molecular Function: tumor necrosis factor receptor activity; receptor activity

Biological Process: regulation of apoptosis; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; apoptosis; signal transduction