Rabbit anti-Human TNFRSF10D Polyclonal Antibody | anti-TNFRSF10D antibody

TNFRSF10D, ID (TNFRSF10D, DCR2, TRAILR4, TRUNDD, Tumor necrosis factor receptor superfamily member 10D, Decoy receptor 2, TNF-related apoptosis-inducing ligand receptor 4, TRAIL receptor with a truncated death domain, CD264) (PE)

Cat. Num. AAA6356921

• Gene Names: TNFRSF10D; DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• Reactivity: Human
• Applications: Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: TNFRSF10D; Polyclonal Antibody; ID (TNFRSF10D; DCR2; TRAILR4; TRUNDD; Tumor necrosis factor receptor superfamily member 10D; Decoy receptor 2; TNF-related apoptosis-inducing ligand receptor 4; TRAIL receptor with a truncated death domain; CD264) (PE); CD264; anti-TNFRSF10D antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TNFRSF10D.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TNFRSF10D antibody: Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 252-278 in the central region of human TNFRSF10D.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HL-60 cell line lysates (35ug/lane) usingMBS648685. TNFRSF10D (arrow) was detected usingMBS648685.)

Flow Cytometry (FC/FACS)

(Flow Cytometry analysis of Hela cells using MBS648685 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TNFRSF10D antibody

TNFRSF is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis.

Product Categories/Family for anti-TNFRSF10D antibody

Antibodies; Apoptosis

References

1. Davila, S., et al. Genes Immun. (2010). In press: 2. Pei, G.T., et al. Biochem. Biophys. Res. Commun. 391(2):1274-1279(2010). 3. Lucas, H., et al. J. Dent. Res. 89(1):29-33(2010). 4. Hosgood, H.D. III, et al. Occup Environ Med 66(12):848-853(2009). 5. Chen, B., et al. Spine 34 (19), E677-E681 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 22547108
• NCBI GeneID: 8793
• NCBI Accession #: NP_003831.2
• NCBI GenBank Nucleotide #: NM_003840.4
• UniProt Accession #: Q9UBN6; Q9Y6Q4; B2R8W0
• Molecular Weight: 41,823 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 10D
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 10d, decoy with truncated death domain
• NCBI Official Symbol: TNFRSF10D
• NCBI Official Synonym Symbols: DCR2; CD264; TRUNDD; TRAILR4; TRAIL-R4
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10D; TRAIL receptor 4; decoy receptor 2; TNF receptor-related receptor for TRAIL; TRAIL receptor with a truncated death domain; TNF-related apoptosis-inducing ligand receptor 4
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10D
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: TNFRSF10D
• UniProt Synonym Gene Names: DCR2; TRAILR4; TRUNDD; DcR2; TRAIL receptor 4; TRAIL-R4
• UniProt Entry Name: TR10D_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain, a transmembrane domain, and a truncated cytoplamic death domain. This receptor does not induce apoptosis, and has been shown to play an inhibitory role in TRAIL-induced cell apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R4: Receptor for the cytotoxic ligand TRAIL. Contains a truncated death domain and hence is not capable of inducing apoptosis but protects against TRAIL-mediated apoptosis. Reports are contradictory with regards to its ability to induce the NF- kappa-B pathway. According to PubMed:9382840, it cannot but according to PubMed:9430226, it can induce the NF-kappa-B pathway. Widely expressed, in particular in fetal kidney, lung and liver, and in adult testis and liver. Also expressed in peripheral blood leukocytes, colon and small intestine, ovary, prostate, thymus, spleen, pancreas, kidney, lung, placenta and heart.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: integral to membrane

Molecular Function: transmembrane receptor activity; TRAIL binding

Biological Process: apoptosis; signal transduction; negative regulation of apoptosis