Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using TNFAIP8L2 antibody.)
Rabbit TNFAIP8L2 Polyclonal Antibody | anti-TNFAIP8L2 antibody
TNFAIP8L2 Antibody
Gene Names
TNFAIP8L2; TIPE2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
TNFAIP8L2; Polyclonal Antibody; TNFAIP8L2 Antibody; AW610835; FLJ23467; Inflammation factor 20; Inflammation factor protein 20; TIPE2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2; TP8L2_HUMAN; Tumor necrosis factor alpha induced protein 8 like 2; Tumor necrosis factor alpha-induced protein 8-like protein 2; anti-TNFAIP8L2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
TNFAIP8L2 antibody detects endogenous levels of total TNFAIP8L2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
184
Applicable Applications for anti-TNFAIP8L2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (100%), Bovine (91%), Horse (100%), Sheep (91%), Rabbit (100%), Dog (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using TNFAIP8L2 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HuvEc cells, using TNFAIP8L2 antibody.)
Immunofluorescene (IF)
(MBS9604787 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9604787 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-TNFAIP8L2 antibody
Function: Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Negative regulator of Toll-like receptor and T-cell receptor function. Prevents hyperresponsiveness of the immune system and maintains immune homeostasis. Inhibits JUN/AP1 and NF-kappa-B activation. Promotes Fas-induced apoptosis (By similarity).
Subunit Structure: May interact with CASP8; however, such result is unclear since PubMed:19079267 could not reproduce the interaction with CASP8.
Similarity: The central region was initially thought to constitute a DED (death effector) domain. However, 3D-structure data reveal a previously uncharacterized fold that is different from the predicted fold of a DED (death effector) domain. It consists of a large, hydrophobic central cavity that is poised for cofactor binding. Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.
Subunit Structure: May interact with CASP8; however, such result is unclear since PubMed:19079267 could not reproduce the interaction with CASP8.
Similarity: The central region was initially thought to constitute a DED (death effector) domain. However, 3D-structure data reveal a previously uncharacterized fold that is different from the predicted fold of a DED (death effector) domain. It consists of a large, hydrophobic central cavity that is poised for cofactor binding. Belongs to the TNFAIP8 family. TNFAIP8L2 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 20 kD
Predicted: 21 kDa
Predicted: 21 kDa
NCBI Official Full Name
tumor necrosis factor alpha-induced protein 8-like protein 2
NCBI Official Synonym Full Names
TNF alpha induced protein 8 like 2
NCBI Official Symbol
TNFAIP8L2
NCBI Official Synonym Symbols
TIPE2
NCBI Protein Information
tumor necrosis factor alpha-induced protein 8-like protein 2
UniProt Protein Name
Tumor necrosis factor alpha-induced protein 8-like protein 2
Protein Family
UniProt Gene Name
TNFAIP8L2
UniProt Synonym Gene Names
TIPE2; TNF alpha-induced protein 8-like protein 2; TNFAIP8-like protein 2
Uniprot Description
Acts as a negative regulator of innate and adaptive immunity by maintaining immune homeostasis. Negative regulator of Toll-like receptor and T-cell receptor function. Prevents hyperresponsiveness of the immune system and maintains immune homeostasis. Inhibits JUN/AP1 and NF-kappa-B activation. Promotes Fas-induced apoptosis ().
Research Articles on TNFAIP8L2
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Product Notes
The TNFAIP8L2 tnfaip8l2 (Catalog #AAA9604787) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TNFAIP8L2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNFAIP8L2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the TNFAIP8L2 tnfaip8l2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNFAIP8L2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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