Rabbit anti-Human TMPRSS2 Polyclonal Antibody | anti-TMPRSS2 antibody

TMPRSS2, ID (Transmembrane Protease, Serine 2, Serine Protease 10, Transmembrane Protease, Serine 2 Non-catalytic Chain, Transmembrane Protease, Serine 2 Catalytic Chain, PRSS10) (MaxLight 650)

Cat. Num. AAA6503462

• Gene Names: TMPRSS2; PP9284; PRSS10
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: TMPRSS2; Polyclonal Antibody; ID (Transmembrane Protease; Serine 2; Serine Protease 10; Transmembrane Protease; Serine 2 Non-catalytic Chain; Serine 2 Catalytic Chain; PRSS10) (MaxLight 650); anti-TMPRSS2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TMPRSS2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-TMPRSS2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human TMPRSS2 (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of TMPRSS2 antibody (Center) in HepG2 cell line lysates (35ug/lane). TMPRSS2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(TMPRSS2 Antibody (Center) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-TMPRSS2 antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

1. Gopalan, A., Cancer Res. 69(4), 1400-1406 (2009). 2. Hofer, M.D., Cancer Res. 69(2), 640-646 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 205360943
• NCBI GeneID: 7113
• NCBI Accession #: NP_005647
• NCBI GenBank Nucleotide #: NM_005656.3
• UniProt Accession #: O15393
• Molecular Weight: 54kDa
• NCBI Official Full Name: transmembrane protease serine 2 isoform 2
• NCBI Official Synonym Full Names: transmembrane serine protease 2
• NCBI Official Symbol: TMPRSS2
• NCBI Official Synonym Symbols: PP9284; PRSS10
• NCBI Protein Information: transmembrane protease serine 2
• UniProt Protein Name: Transmembrane protease serine 2
• Protein Family: Transmembrane protease serine
• UniProt Gene Name: TMPRSS2
• UniProt Synonym Gene Names: PRSS10

NCBI Description

This gene encodes a protein that belongs to the serine protease family. The encoded protein contains a type II transmembrane domain, a receptor class A domain, a scavenger receptor cysteine-rich domain and a protease domain. Serine proteases are known to be involved in many physiological and pathological processes. This gene was demonstrated to be up-regulated by androgenic hormones in prostate cancer cells and down-regulated in androgen-independent prostate cancer tissue. The protease domain of this protein is thought to be cleaved and secreted into cell media after autocleavage. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

Serine protease that proteolytically cleaves and activates the viral spike glycoproteins which facilitate virus-cell membrane fusions; spike proteins are synthesized and maintained in precursor intermediate folding states and proteolysis permits the refolding and energy release required to create stable virus-cell linkages and membrane coalescence. Facilitates human SARS coronavirus (SARS-CoV) infection via two independent mechanisms, proteolytic cleavage of ACE2, which might promote viral uptake, and cleavage of coronavirus spike glycoprotein which activates the glycoprotein for cathepsin L-independent host cell entry. Proteolytically cleaves and activates the spike glycoproteins of human coronavirus 229E (HCoV-229E) and human coronavirus EMC (HCoV-EMC) and the fusion glycoproteins F0 of Sendai virus (SeV), human metapneumovirus (HMPV), human parainfluenza 1, 2, 3, 4a and 4b viruses (HPIV). Essential for spread and pathogenesis of influenza A virus (strains H1N1, H3N2 and H7N9); involved in proteolytic cleavage and activation of hemagglutinin (HA) protein which is essential for viral infectivity.