Western Blot (WB)
(TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)
Rabbit anti-Human, Mouse TMEM66 Polyclonal Antibody | anti-TMEM66 antibody
TMEM66, NT (TMEM66, SARAF, XTP3, Store-operated calcium entry-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66) (FITC)
Gene Names
SARAF; XTP3; FOAP-7; TMEM66; HSPC035
Reactivity
Human, Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TMEM66; Polyclonal Antibody; NT (TMEM66; SARAF; XTP3; Store-operated calcium entry-associated regulatory factor; HBV X-transactivated gene 3 protein; HBV XAg-transactivated protein 3; Protein FOAP-7; Transmembrane protein 66) (FITC); Transmembrane protein 66; anti-TMEM66 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-TMEM66 antibody
Flow Cytometry (FC/FACS), FLISA, Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
TMEM66 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids from the N-terminal region of human TMEM66.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)
Western Blot (WB)
(TMEM66 Antibody (N-term) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)
Western Blot (WB)
(TMEM66 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)
Western Blot (WB)
(TMEM66 Antibody (N-term) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TMEM66 antibody detected the TMEM66 protein (arrow).)
Flow Cytometry (FC/FACS)
(TMEM66 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(TMEM66 Antibody (N-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-TMEM66 antibody
TMEM66 is a multi-pass membrane protein which belongs to the TMEM66 family. The exact function of TMEM66 remains unknown.
Product Categories/Family for anti-TMEM66 antibody
References
Rose, J. Phd, et al. Mol. Med. (2010). In press: Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004). Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36975
NCBI Official Full Name
store-operated calcium entry-associated regulatory factor isoform 1
NCBI Official Synonym Full Names
store-operated calcium entry-associated regulatory factor
NCBI Official Symbol
SARAF
NCBI Official Synonym Symbols
XTP3; FOAP-7; TMEM66; HSPC035
NCBI Protein Information
store-operated calcium entry-associated regulatory factor
UniProt Protein Name
Store-operated calcium entry-associated regulatory factor
UniProt Gene Name
SARAF
UniProt Synonym Gene Names
TMEM66; XTP3; SARAF; SOCE-associated regulatory factor
UniProt Entry Name
SARAF_HUMAN
Research Articles on TMEM66
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Product Notes
The TMEM66 saraf (Catalog #AAA6356507) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TMEM66, NT (TMEM66, SARAF, XTP3, Store-operated calcium entry-associated regulatory factor, HBV X-transactivated gene 3 protein, HBV XAg-transactivated protein 3, Protein FOAP-7, Transmembrane protein 66) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TMEM66 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TMEM66 saraf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TMEM66, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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