Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KRAS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Rabbit KRAS Polyclonal Antibody | anti-KRAS antibody
KRAS Polyclonal Antibody
IF: 1:50-1:200
IP: 1:50-1:200
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KRAS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KRAS antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (MBS9132556) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min)
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and KRAS knockout (KO) 293T cells, using KRAS antibody (MBS9132556) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KRAS antibody (MBS9132556) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using KRAS antibody (MBS9132556) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Western Blot (WB)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug KRAS antibody (MBS9132556).Western blot was performed from the immunoprecipitate using KRAS antibody (MBS9132556) at a dilition of 1:1000.)
Western Blot (WB)
(Immunoprecipitation analysis of 600ug extracts of Mouse brain cells using 3ug KRAS antibody (MBS9132556).Western blot was performed from the immunoprecipitate using KRAS antibody (MBS9132556) at a dilition of 1:1000.)
NCBI and Uniprot Product Information
Calculated MW: 21kD
NCBI Description
This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]
Uniprot Description
KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein
Chromosomal Location of Human Ortholog: 12p12.1
Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane; focal adhesion; membrane; plasma membrane
Molecular Function: protein binding; protein complex binding
Biological Process: axon guidance; epidermal growth factor receptor signaling pathway; leukocyte migration; MAPKKK cascade; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; Ras protein signal transduction; regulation of protein stability; stimulatory C-type lectin receptor signaling pathway
Disease: Bladder Cancer; Breast Cancer; Cardiofaciocutaneous Syndrome 2; Gastric Cancer, Hereditary Diffuse; Leukemia, Acute Myeloid; Lung Cancer; Noonan Syndrome 3; Pancreatic Cancer; Schimmelpenning-feuerstein-mims Syndrome
Research Articles on KRAS
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Product Notes
The KRAS kras (Catalog #AAA9132556) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRAS Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KRAS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500 - 1:2000 IF: 1:50-1:200 IP: 1:50-1:200. Researchers should empirically determine the suitability of the KRAS kras for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRAS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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