Rabbit anti-Human TMC8 Polyclonal Antibody | anti-TMC8 antibody

TMC8, NT (TMC8, EVER2, EVIN2, Transmembrane channel-like protein 8, Epidermodysplasia verruciformis protein 2) (APC)

Cat. Num. AAA6356164

• Gene Names: TMC8; EV2; EVER2; EVIN2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TMC8; Polyclonal Antibody; NT (TMC8; EVER2; EVIN2; Transmembrane channel-like protein 8; Epidermodysplasia verruciformis protein 2) (APC); Epidermodysplasia verruciformis protein 2; anti-TMC8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-TMC8 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TMC8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human TMC8.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TMC8 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the TMC8 antibody detected the TMC8 protein (arrow).)

Related Product Information for anti-TMC8 antibody

Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 8 predicted transmembrane domains and 3 leucine zipper motifs.

Product Categories/Family for anti-TMC8 antibody

Antibodies; Transmembrane Proteins

References

McDermott, D.F., et al. Pediatr Dermatol 26(3):306-310(2009). Patel, A.S., et al. Int. J. Cancer 122(10):2377-2379(2008). Zavattaro, E., et al. J. Invest. Dermatol. 128(3):732-735(2008). Lazarczyk, M., et al. J. Exp. Med. 205(1):35-42(2008). Rady, P.L., et al. Br. J. Dermatol. 157(4):831-833(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 23943900
• NCBI GeneID: 147138
• NCBI Accession #: NP_689681.2
• NCBI GenBank Nucleotide #: NM_152468.4
• UniProt Accession #: Q8IU68; Q2YDC0; Q8IWU7; Q8N358; Q8NF04
• Molecular Weight: 81,641 Da
• NCBI Official Full Name: transmembrane channel-like protein 8
• NCBI Official Synonym Full Names: transmembrane channel-like 8
• NCBI Official Symbol: TMC8
• NCBI Official Synonym Symbols: EV2; EVER2; EVIN2
• NCBI Protein Information: transmembrane channel-like protein 8; epidermodysplasia verruciformis 2; epidermodysplasia verruciformis protein 2
• UniProt Protein Name: Transmembrane channel-like protein 8
• Protein Family: Transmembrane channel-like protein
• UniProt Gene Name: TMC8
• UniProt Synonym Gene Names: EVER2; EVIN2
• UniProt Entry Name: TMC8_HUMAN

NCBI Description

Epidermodysplasia verruciformis (EV) is an autosomal recessive dermatosis characterized by abnormal susceptibility to human papillomaviruses (HPVs) and a high rate of progression to squamous cell carcinoma on sun-exposed skin. EV is caused by mutations in either of two adjacent genes located on chromosome 17q25.3. Both of these genes encode integral membrane proteins that localize to the endoplasmic reticulum and are predicted to form transmembrane channels. This gene encodes a transmembrane channel-like protein with 8 predicted transmembrane domains and 3 leucine zipper motifs. [provided by RefSeq, Jul 2008]

Uniprot Description

TMC8: an endoplasmic reticulum multipass membrane protein. Defects in TMC8 cause Lutz-Lewandowsky epidermodysplasia verruciformis [MIM:226400], a rare skin disease characterized by increased susceptibility to infection by papillomaviruses with an accompanying high risk of skin carcinoma. Three splice-variant isoforms of the human protein have been described.

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; nuclear membrane; endoplasmic reticulum; cytoplasm; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: negative regulation of protein oligomerization; zinc ion homeostasis; ion transport; regulation of cell growth; negative regulation of protein binding

Disease: Epidermodysplasia Verruciformis