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Western Blot (WB) (TM181 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TM181 antibody detected the TM181 protein (arrow).)

Rabbit anti-Human TM181 Polyclonal Antibody | anti-TM181 antibody

TM181, NT (TMEM181, GPR178, KIAA1423, Transmembrane protein 181) (AP)

Gene Names
TMEM181; GPR178; KIAA1423
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
TM181; Polyclonal Antibody; NT (TMEM181; GPR178; KIAA1423; Transmembrane protein 181) (AP); TMEM181; Transmembrane protein 181; anti-TM181 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-TM181 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
TM181 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the N-terminal region of human TM181.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TM181 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TM181 antibody detected the TM181 protein (arrow).)

Western Blot (WB) (TM181 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TM181 antibody detected the TM181 protein (arrow).)

Immunohistochemistry (IHC)

(TM181 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TM181 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (TM181 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TM181 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-TM181 antibody
The TMEM181 gene encodes a putative G protein-coupled receptor expressed on the cell surface (Carette et al., 2009 [PubMed 19965467]; Wollscheid et al., 2009 [PubMed 19349973]).
Product Categories/Family for anti-TM181 antibody
References
Carette, J.E., et al. Science 326(5957):1231-1235(2009). Wollscheid, B., et al. Nat. Biotechnol. 27(4):378-386(2009). Olsen, J.V., et al. Cell 127(3):635-648(2006). Wistrand, M., et al. Protein Sci. 15(3):509-521(2006). Cheng, J., et al. Science 308(5725):1149-1154(2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,325 Da
NCBI Official Full Name
transmembrane protein 181
NCBI Official Synonym Full Names
transmembrane protein 181
NCBI Official Symbol
TMEM181
NCBI Official Synonym Symbols
GPR178; KIAA1423
NCBI Protein Information
transmembrane protein 181; G protein-coupled receptor 178
UniProt Protein Name
Transmembrane protein 181
UniProt Gene Name
TMEM181
UniProt Synonym Gene Names
GPR178; KIAA1423
UniProt Entry Name
TM181_HUMAN

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Product Notes

The TM181 tmem181 (Catalog #AAA6356020) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TM181, NT (TMEM181, GPR178, KIAA1423, Transmembrane protein 181) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TM181 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TM181 tmem181 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TM181, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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