Rabbit anti-Human GPSM1 Polyclonal Antibody | anti-GPSM1 antibody

GPSM1, ID (GPSM1, AGS3, G-protein-signaling modulator 1, Activator of G-protein signaling 3) (AP)

Cat. Num. AAA6304151

• Gene Names: GPSM1; AGS3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: GPSM1; Polyclonal Antibody; ID (GPSM1; AGS3; G-protein-signaling modulator 1; Activator of G-protein signaling 3) (AP); Activator of G-protein signaling 3; anti-GPSM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 675

• Applicable Applications for anti-GPSM1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: GPSM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 446-476 amino acids from the Central region of human GPSM1.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(GPSM1 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the GPSM1 antibody detected the GPSM1 protein (arrow).)

Immunohistochemistry (IHC)

(GPSM1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GPSM1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-GPSM1 antibody

G proteins propagate intracellular signals initiated by G protein-coupled receptors. GPSM1, a receptor-independent activator of G protein signaling, is one of several factors that influence the basal activity of G protein signaling systems (Pizzinat et al., 2001 [PubMed 11278352]).

Product Categories/Family for anti-GPSM1 antibody

Antibodies; G Proteins

References

Oner, S.S., et al. J. Biol. Chem. 285(44):33949-33958(2010). Davila, S., et al. Genes Immun. 11(3):232-238(2010). Vural, A., et al. Mol. Cell. Biol. 30(6):1528-1540(2010). Groves, B., et al. PLoS ONE 5 (1), E8877 (2010): An, N., et al. J. Biol. Chem. 283(36):24718-24728(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 224548927
• NCBI GeneID: 26086
• NCBI Accession #: NP_001139110.1
• NCBI GenBank Nucleotide #: NP_001139110.1
• UniProt Accession #: Q86YR5
• NCBI Official Full Name: G-protein-signaling modulator 1 isoform a
• NCBI Official Synonym Full Names: G protein signaling modulator 1
• NCBI Official Symbol: GPSM1
• NCBI Official Synonym Symbols: AGS3
• NCBI Protein Information: G-protein-signaling modulator 1
• UniProt Protein Name: G-protein-signaling modulator 1
• Protein Family: G-protein-signaling modulator
• UniProt Gene Name: GPSM1
• UniProt Synonym Gene Names: AGS3
• UniProt Entry Name: GPSM1_HUMAN

NCBI Description

G-protein signaling modulators (GPSMs) play diverse functional roles through their interaction with G-protein subunits. This gene encodes a receptor-independent activator of G protein signaling, which is one of several factors that influence the basal activity of G-protein signaling systems. The protein contains seven tetratricopeptide repeats in its N-terminal half and four G-protein regulatory (GPR) motifs in its C-terminal half. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Function: Guanine nucleotide dissociation inhibitor (GDI) which functions as a receptor-independent activator of heterotrimeric G-protein signaling. Keeps G(i/o) alpha subunit in its GDP-bound form thus uncoupling heterotrimeric G-proteins signaling from G protein-coupled receptors. Controls spindle orientation and asymmetric cell fate of cerebral cortical progenitors. May also be involved in macroautophagy in intestinal cells. May play a role in drug addiction. Ref.6 Ref.7

Subunit structure: Interacts with GNAI1, GNAI2 and GNAI3 preferentially in their GDP-bound state. May also interact with GNAO1. Interacts with STK11/LKB1 and MACF1

By similarity. Interacts with INSC/inscuteable and FRMPD1. Ref.8 Ref.9

Subcellular location: Cytoplasm › cytosol. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side Ref.7 Ref.9.

Tissue specificity: Expressed in intestinal cells. Ref.7

Domain: The GoLoco domains mediate interaction with G(i/o) alpha

By similarity. The GoLoco domains are essential for the GDI activity toward G(i/o) alpha.

Post-translational modification: Phosphorylation regulates interaction with G(i/o) alpha

By similarity.

Sequence similarities: Belongs to the GPSM family.Contains 4 GoLoco domains.Contains 9 TPR repeats.

Sequence caution: The sequence AAH09979.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence AAH17353.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.The sequence CAB55951.1 differs from that shown. Reason: Frameshift at position 657. The sequence CAI19339.1 differs from that shown. Reason: Erroneous gene model prediction.